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Journal of Clinical Microbiology, December 2001, p. 4563-4565, Vol. 39, No. 12
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.12.4563-4565.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Rapid PCR-Single-Strand Conformation Polymorphism Method To Differentiate and Estimate Relative Abundance of Pneumocystis carinii Special Forms Infecting Rats

Aimable Nahimana,1 Melanie T. Cushion,2 Dominique S. Blanc,1 and Philippe M. Hauser1,*

Division Autonome de Médecine Préventive Hospitalière, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland,1 and Division of Infectious Disease, University of Cincinnati College of Medicine, and Cincinnati Veterans Administration Medical Center, Cincinnati, Ohio2

Received 23 May 2001/Returned for modification 20 September 2001/Accepted 28 September 2001

A rapid method that uses PCR-single-strand conformation polymorphism analysis of the intron of the nuclear 26S rRNA gene was shown to differentiate the two Pneumocystis carinii special forms that infect rats, P. carinii f. sp. carinii and P. carinii f. sp. ratti. The method also provides a means for estimation of the relative abundance of the two special forms in the case of a coinfected rat. The results suggest that the method described will help to further standardize the immunosuppressed rat model of P. carinii infection and, thus, contribute to a better understanding of P. carinii infection in humans.

* Corresponding author. Mailing address: Centre Hospitalier Universitaire Vaudois, Division de Médecine Préventive Hospitalière, IMU 218, av. du Bugnon 44, 1011 Lausanne, Switzerland. Phone: 41 21 314 02 68. Fax: 41 21 314 40 60. E-mail: Philippe.Hauser{at}chuv.hospvd.ch.

Journal of Clinical Microbiology, December 2001, p. 4563-4565, Vol. 39, No. 12
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.12.4563-4565.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Cushion, M. T., Keely, S. P., Stringer, J. R. (2004). Molecular and phenotypic description of Pneumocystis wakefieldiae sp. nov., a new species in rats. Mycologia 96: 429-438 [Abstract] [Full Text]  


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