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Journal of Clinical Microbiology, December 2001, p. 4577-4578, Vol. 39, No. 12
Department of Clinical Sciences, College of
Veterinary Medicine, North Carolina State University, Raleigh,
North Carolina 27606-1428
Received 20 April 2001/Returned for modification 15 September
2001/Accepted 26 September 2001
We have encountered a previously unrecognized specificity problem
when using the small-subunit ribosomal DNA (16S rDNA)-based PCR primers
recommended for use in the identification of Ehrlichia equi in clinical samples. These PCR primers annealed to
E. platys 16S rDNA in blood samples containing high
levels of E. platys organisms. Therefore, we designed
and tested new PCR primers for the identification of E.
equi.
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.12.4577-4578.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Differentiation of Ehrlichia platys
and E. equi Infections in Dogs by Using 16S Ribosomal
DNA-Based PCR
*
Corresponding author. Mailing address: Department of
Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough St., Raleigh, NC 27606-1428. Phone: (919)
513-6433. Fax: (919) 513-6336. E-mail:
Christian_Pitulle{at}ncsu.edu.
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