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Journal of Clinical Microbiology, December 2001, p. 4577-4578, Vol. 39, No. 12
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.12.4577-4578.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Differentiation of Ehrlichia platys and E. equi Infections in Dogs by Using 16S Ribosomal DNA-Based PCR

Susan I. Hancock, Edward B. Breitschwerdt, and Christian Pitulle*

Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27606-1428

Received 20 April 2001/Returned for modification 15 September 2001/Accepted 26 September 2001

We have encountered a previously unrecognized specificity problem when using the small-subunit ribosomal DNA (16S rDNA)-based PCR primers recommended for use in the identification of Ehrlichia equi in clinical samples. These PCR primers annealed to E. platys 16S rDNA in blood samples containing high levels of E. platys organisms. Therefore, we designed and tested new PCR primers for the identification of E. equi.


* Corresponding author. Mailing address: Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough St., Raleigh, NC 27606-1428. Phone: (919) 513-6433. Fax: (919) 513-6336. E-mail: Christian_Pitulle{at}ncsu.edu.


Journal of Clinical Microbiology, December 2001, p. 4577-4578, Vol. 39, No. 12
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.12.4577-4578.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.



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