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Journal of Clinical Microbiology, February 2001, p. 539-543, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.539-543.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Immunodiagnostic Identification of Dairy Cows Infected with Prototheca zopfii at Various Clinical Stages and Discrimination between Infected and Uninfected Cows

Uwe Roesler,* Holger Scholz, and Andreas Hensel

Institute of Animal Hygiene and Veterinary Public Health, Faculty of Veterinary Medicine, University of Leipzig, D-04103 Leipzig, Germany

Received 3 July 2000/Returned for modification 26 September 2000/Accepted 17 November 2000

Protothecosis is a severe form of mastitis in cattle that is caused by colorless algae of the genus Prototheca. So far, no suitable serological test for the identification of infected animals is available for routine diagnosis. In this study an indirect enzyme-linked immunosorbent assay (ELISA) for the identification of infected cows and for discriminating among infected cows at various clinical stages was developed. Immunoglobulin G (IgG) in serum and IgA and IgG1 in whey were used as antibody isotypes. The ELISA was evaluated using serum and whey from animals at different clinical stages of infection. A total of 12 cows with acute clinical manifestation of protothecal mastitis, 22 cows with clinical signs of chronic mastitis, 40 Prototheca zopfii-negative cows, and 18 cows with chronic clinical signs and earlier cultures positive for P. zopfii but with presently negative culturing results were investigated. A sensitivity of 96% and a specificity of 94% were calculated for the ELISA based on IgA levels. Intra-assay and interassay variations were calculated to be 6.08 and 6.32%, respectively. Based on these data, this ELISA was found to be suitable for discrimination between infected and uninfected animals and might therefore be useful for screening affected herds.

* Corresponding author. Mailing address: Institut für Tierhygiene und Öffentliches Veterinärwesen, Veterinärmedizinische Fakultät, Universität Leipzig, An den Tierkliniken 43, D-04103 Leipzig, Germany. Phone: 49-341-97-38150. Fax: 49-341-97-38198. E-mail: roesler{at}vetmed.uni-leipzig.de.

Journal of Clinical Microbiology, February 2001, p. 539-543, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.539-543.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Lass-Florl, C., Mayr, A. (2007). Human Protothecosis. Clin. Microbiol. Rev. 20: 230-242 [Abstract] [Full Text]  
  • Marques, S., Silva, E., Carvalheira, J., Thompson, G. (2006). Short communication: In vitro antimicrobial susceptibility of Prototheca wickerhamii and Prototheca zopfii isolated from bovine mastitis.. J DAIRY SCI 89: 4202-4204 [Abstract] [Full Text]  
  • Roesler, U., Moller, A., Hensel, A., Baumann, D., Truyen, U. (2006). Diversity within the current algal species Prototheca zopfii: a proposal for two Prototheca zopfii genotypes and description of a novel species, Prototheca blaschkeae sp. nov.. Int. J. Syst. Evol. Microbiol. 56: 1419-1425 [Abstract] [Full Text]  
  • Roesler, U., Scholz, H., Hensel, A. (2003). Emended phenotypic characterization of Prototheca zopfii: a proposal for three biotypes and standards for their identification. Int. J. Syst. Evol. Microbiol. 53: 1195-1199 [Abstract] [Full Text]  
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