This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hennequin, C.
Right arrow Articles by Dujon, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hennequin, C.
Right arrow Articles by Dujon, B.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, February 2001, p. 551-559, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.551-559.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Microsatellite Typing as a New Tool for Identification of Saccharomyces cerevisiae Strains

C. Hennequin,1,2,* A. Thierry,2 G. F. Richard,2 G. Lecointre,3 H. V. Nguyen,4 C. Gaillardin,4 and B. Dujon2

Service de Parasitologie-Mycologie et Médecine des Voyages, CHU Amiens, F-80054 Amiens,1 Unité de Génétique Moléculaire des Levures, URA 2171 CNRS and UFR 927, Université Pierre et Marie Curie, Paris, Institut Pasteur, F-75724 Paris,2 Service de Systematique Moléculaire (CNRS GDR 1005), Laboratoire d'Ichtyologie Generale et Appliquee, Museum National d'Histoire Naturelle, 75231 Paris Cedex 05,3 and Collection de Levures d'Intérêt Biotechnologique, UMR 216, Microbiologie et Génétique Moléculaire, Institut National d'Agronomie de Paris Grignon, F-78850 Thiverval Grignon,4 France

Received 25 July 2000/Returned for modification 14 August 2000/Accepted 12 October 2000

Since Saccharomyces cerevisiae appears to be an emerging pathogen, there is a need for a valuable molecular marker able to distinguish among strains. In this work, we investigated the potential value of microsatellite length polymorphism with a panel of 91 isolates, including 41 clinical isolates, 14 laboratory strains, and 28 strains with industrial relevance. Testing seven polymorphic regions (five trinucleotide repeats and two dinucleotide repeats) in a subgroup of 58 unrelated strains identified a total of 69 alleles (6 to 13 per locus) giving 52 different patterns with a discriminatory power of 99.03%. We found a cluster of clinical isolates sharing their genotype with a bakery strain, suggesting a digestive colonization following ingestion of this strain with diet. With the exception of this cluster of isolates and isolates collected from the same patient or from patients treated with Saccharomyces boulardii, all clinical isolates gave different and unique patterns. The genotypes are stable, and the method is reproducible. The possibility to make the method portable is of great interest for further studies using this technique. This work shows the possibility to readily identify S. boulardii (a strain increasingly isolated from invasive infections) using a unique and specific microsatellite allele.

* Corresponding author. Mailing address: Unité de Génétique Moléculaire des Levures, URA 2171 CNRS and UFR 927, Université Pierre et Marie Curie, Paris, Institut Pasteur, 25 rue du Dr. Roux, 75724 Paris, France. Phone: 33-1-45-68-07. Fax: 33-1-40-61-34-56. E-mail: chennequin{at}yahoo.com.

Journal of Clinical Microbiology, February 2001, p. 551-559, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.551-559.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Richard, G.-F., Kerrest, A., Dujon, B. (2008). Comparative Genomics and Molecular Dynamics of DNA Repeats in Eukaryotes. Microbiol. Mol. Biol. Rev. 72: 686-727 [Abstract] [Full Text]  
  • Oliveira, V. A., Vicente, M. A., Fietto, L. G., de Miranda Castro, I., Coutrim, M. X., Schuller, D., Alves, H., Casal, M., de Oliveira Santos, J., Araujo, L. D., da Silva, P. H. A., Brandao, R. L. (2008). Biochemical and Molecular Characterization of Saccharomyces cerevisiae Strains Obtained from Sugar-Cane Juice Fermentations and Their Impact in Cachaca Production. Appl. Environ. Microbiol. 74: 693-701 [Abstract] [Full Text]  
  • Grenouillet, F., Millon, L., Bart, J.-M., Roussel, S., Biot, I., Didier, E., Ong, A.-S., Piarroux, R. (2007). Multiple-Locus Variable-Number Tandem-Repeat Analysis for Rapid Typing of Candida glabrata. J. Clin. Microbiol. 45: 3781-3784 [Abstract] [Full Text]  
  • Edwards-Ingram, L., Gitsham, P., Burton, N., Warhurst, G., Clarke, I., Hoyle, D., Oliver, S. G., Stateva, L. (2007). Genotypic and Physiological Characterization of Saccharomyces boulardii, the Probiotic Strain of Saccharomyces cerevisiae. Appl. Environ. Microbiol. 73: 2458-2467 [Abstract] [Full Text]  
  • Matute, D. R., Sepulveda, V. E., Quesada, L. M., Goldman, G. H., Taylor, J. W., Restrepo, A., McEwen, J. G. (2006). Microsatellite Analysis of Three Phylogenetic Species of Paracoccidioides brasiliensis.. J. Clin. Microbiol. 44: 2153-2157 [Abstract] [Full Text]  
  • Lasker, B. A., Butler, G., Lott, T. J. (2006). Molecular Genotyping of Candida parapsilosis Group I Clinical Isolates by Analysis of Polymorphic Microsatellite Markers.. J. Clin. Microbiol. 44: 750-759 [Abstract] [Full Text]  
  • Richard, G.-F., Dujon, B. (2006). Molecular Evolution of Minisatellites in Hemiascomycetous Yeasts. Mol Biol Evol 23: 189-202 [Abstract] [Full Text]  
  • Sampaio, P., Gusmao, L., Correia, A., Alves, C., Rodrigues, A. G., Pina-Vaz, C., Amorim, A., Pais, C. (2005). New Microsatellite Multiplex PCR for Candida albicans Strain Typing Reveals Microevolutionary Changes. J. Clin. Microbiol. 43: 3869-3876 [Abstract] [Full Text]  
  • Malgoire, J. Y., Bertout, S., Renaud, F., Bastide, J. M., Mallie, M. (2005). Typing of Saccharomyces cerevisiae Clinical Strains by Using Microsatellite Sequence Polymorphism. J. Clin. Microbiol. 43: 1133-1137 [Abstract] [Full Text]  
  • Nascimento, E., Martinez, R., Rodrigues Lopes, A., de Souza Bernardes, L. A., Pomponio Barco, C., Goldman, M. H. S., Taylor, J. W., McEwen, J. G., Pasetto Nobrega, M., Nobrega, F. G., Goldman, G. H. (2004). Detection and Selection of Microsatellites in the Genome of Paracoccidioides brasiliensis as Molecular Markers for Clinical and Epidemiological Studies. J. Clin. Microbiol. 42: 5007-5014 [Abstract] [Full Text]  
  • Edwards-Ingram, L. C., Gent, M. E., Hoyle, D. C., Hayes, A., Stateva, L. I., Oliver, S. G. (2004). Comparative Genomic Hybridization Provides New Insights Into the Molecular Taxonomy of the Saccharomyces Sensu Stricto Complex. Genome Res 14: 1043-1051 [Abstract] [Full Text]  
  • Lasker, B. A., Ran, Y. (2004). Analysis of Polymorphic Microsatellite Markers for Typing Penicillium marneffei Isolates. J. Clin. Microbiol. 42: 1483-1490 [Abstract] [Full Text]  
  • Sampaio, P., Gusmao, L., Alves, C., Pina-Vaz, C., Amorim, A., Pais, C. (2003). Highly Polymorphic Microsatellite for Identification of Candida albicans Strains. J. Clin. Microbiol. 41: 552-557 [Abstract] [Full Text]  
  • Botterel, F., Desterke, C., Costa, C., Bretagne, S. (2001). Analysis of Microsatellite Markers of Candida albicans Used for Rapid Typing. J. Clin. Microbiol. 39: 4076-4081 [Abstract] [Full Text]  
  • Bart-Delabesse, E., Sarfati, J., Debeaupuis, J.-P., van Leeuwen, W., van Belkum, A., Bretagne, S., Latge, J.-P. (2001). Comparison of Restriction Fragment Length Polymorphism, Microsatellite Length Polymorphism, and Random Amplification of Polymorphic DNA Analyses for Fingerprinting Aspergillus fumigatus Isolates. J. Clin. Microbiol. 39: 2683-2686 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»