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Journal of Clinical Microbiology, February 2001, p. 596-600, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.596-600.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Circulating Nucleic Acids of Chlamydia pneumoniae and Cytomegalovirus in Patients Undergoing Coronary Angiography

M. Smieja,*,1 S. Chong,1 M. Natarajan,2 A. Petrich,1,3 L. Rainen,4 and J. B. Mahony1,3

Hamilton Regional Laboratory Medicine Programme1 and Department of Medicine2 and Department of Pathology and Molecular Medicine,3 McMaster University, Hamilton, Ontario, Canada, and BD Vacutainer Systems, Franklin Lakes, New Jersey4

Received 10 July 2000/Returned for modification 16 October 2000/Accepted 21 November 2000

Peripheral blood mononuclear cells from 208 consecutive patients undergoing elective coronary angiography or angioplasty were collected before, immediately after, and 4 h after the procedure. Nucleic acids of Chlamydia pneumoniae and of cytomegalovirus (CMV) were detected by PCR and confirmed by hybridization. Circulating C. pneumoniae DNA was identified in 24 patients (11.5%) and was associated with current smoking (odds ratio [OR] = 4.5, 95% confidence interval [CI] = 1.6 to 12.2, P = 0.004) but not with arterial narrowing on coronary angiogram or with serological results positive for C. pneumoniae. Circulating CMV DNA was identified in 36 patients (17.3%) and was associated with anti-CMV immunoglobulin G (OR = 2.7, 95% CI = 1.2 to 6.3, P = 0.02) but not with angiographic arterial narrowing or with the need for revascularization. Neither C. pneumoniae nor CMV DNA detection increased after angioplasty, a procedure in which endothelium is disrupted. Larger prospective studies are needed to determine the prognostic significance of DNA detection.

* Corresponding author. Mailing address: Laboratory Medicine L424, St. Joseph's Hospital, 50 Charlton Ave. E., Hamilton, Ontario L8N 4A6, Canada. Phone: (905) 522-1155, ext. 5140. Fax: (905) 521-6083. E-mail: smiejam{at}mcmaster.ca.

Journal of Clinical Microbiology, February 2001, p. 596-600, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.596-600.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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