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Journal of Clinical Microbiology, February 2001, p. 642-646, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.642-646.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Detection and Identification of Eight Trichinella Genotypes by Reverse Line Blot Hybridization

Y. B. Rombout, S. Bosch, and J. W. B. Van Der Giessen*

Microbiological Laboratory for Health Protection, National Institute of Public Health and the Environment (RIVM), 3720 BA Bilthoven, The Netherlands

Received 10 July 2000/Returned for modification 8 October 2000/Accepted 16 November 2000

A reverse line blot (RLB) assay was developed to identify different Trichinella genotypes. The RLB assay accomplishes detection and specific identification of the different Trichinella genotypes and relies on hybridization of the amplified 5S ribosomal DNA intergenic spacer regions to specific, membrane-bound oligonucleotide probes. After one single amplification, we were able to detect and genetically identify six sibling species, i.e., T. spiralis, T. britovi, T. nativa, T. murrelli, T. nelsoni, and T. pseudospiralis, and two additional Trichinella genotypes, T6 and T8. Twenty-four Trichinella strains of different genotypes were unequivocally identified evaluated using one simple PCR-based assay based on single larvae. This assay allows the specific identification of Trichinella species without the need to passage larvae in laboratory animals.

* Corresponding author. Mailing address: Microbiological Laboratory for Health Protection, National Institute of Public Health and the Environment, Antonie van Leeuwenhoeklaan 9, P.O. Box 1, 3720 BA Bilthoven, The Netherlands. Phone: 3130-2743926. Fax: 3130-2744434. E-mail: joke.van.der.giessen{at}rivm.nl.

Journal of Clinical Microbiology, February 2001, p. 642-646, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.642-646.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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