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Journal of Clinical Microbiology, February 2001, p. 691-695, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.691-695.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Rapid Detection of Mutations in the 23S rRNA Gene of Helicobacter pylori That Confers Resistance to Clarithromycin Treatment to the Bacterium

Masayuki Matsumura,1,* Yoko Hikiba,1 Keiji Ogura,1,2 Goichi Togo,2 Izumi Tsukuda,1 Kenji Ushikawa,1 Yasushi Shiratori,2 and Masao Omata2

The Institute for Adult Diseases, Asahi Life Foundation, 1-9-14 Nishi-Shinjuku, Shinjuku-ku, Tokyo 160-0023,1 and Division of Gastroenterology, Department of Internal Medicine, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655,2 Japan

Received Recieved 17 July 2000/Returned for modification 20 September 2000/Accepted 11 November 2000

We developed a new method capable of detecting point mutations in the 23S rRNA gene of Helicobacter pylori using a LightCycler. Our method can detect a mutation in this gene in less than 1 h and can process many samples at once, thereby contributing to the selection of patients suitable for clarithromycin-based therapy.

* Corresponding author. Mailing address: The Institute for Adult Diseases, Asahi Life Foundation, 1-9-14 Nishi-Shinjuku, Shinjuku-ku, Tokyo 160-0023, Japan. Phone: 81-3-3343-2151. Fax: 81-3-3344-6275. E-mail: m-matsumura{at}asahi-life.or.jp.

Journal of Clinical Microbiology, February 2001, p. 691-695, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.691-695.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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