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Journal of Clinical Microbiology, February 2001, p. 716-719, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.716-719.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Development of Monoclonal Antibodies Which Specifically Recognize Entamoeba histolytica in Preserved Stool Samples

Yvonne C. W. Yau,1 Ian Crandall,2,3 and Kevin C. Kain3,*

Department of Medical Genetics and Microbiology1 and Department of Laboratory Medicine and Pathobiology,2 University of Toronto, Toronto, Ontario, Canada M5S 1A8, and Tropical Disease Unit, Division of Infectious Diseases, Department of Medicine, Toronto General Hospital and the University of Toronto, Toronto, Ontario, Canada M5G 2C43

Received 26 July 2000/Returned for modification 28 September 2000/Accepted 17 November 2000

We report the generation of monoclonal antibodies against a recombinant 170-kDa subunit of the Gal or GalNAc lectin of Entamoeba histolytica that specifically recognize E. histolytica but not Entamoeba dispar in preserved stool samples. These antibodies do not cross-react with other bowel protozoa, including Entamoeba coli, Giardia lamblia, and Dientamoeba fragilis.

* Corresponding author. Mailing address: Tropical Disease Unit, EN G-224, The University Health Network, Toronto General Hospital, 200 Elizabeth S., Toronto, ON, Canada M5G 2C4. Phone: (416) 340-3535. Fax: (416) 595-5826. E-mail: Kevin.Kain{at}uhn.on.ca.

Journal of Clinical Microbiology, February 2001, p. 716-719, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.716-719.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.




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