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Journal of Clinical Microbiology, February 2001, p. 738-739, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.738-739.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Use of BACTEC 9240 Blood Culture System for Detection of Brucella melitensis in Synovial Fluid

Pablo Yagupsky,1,* Nechama Peled,1 and Joseph Press2

Clinical Microbiology Laboratories1 and Pediatric Rheumatology Unit,2 Soroka Medical Center, Ben-Gurion University of the Negev, Beer-Sheva, Israel

Received 9 August 2000/Returned for modification 21 October 2000/Accepted 11 November 2000

Synovial fluid specimens aspirated from patients with arthritis were inoculated into an aerobic Peds Plus blood culture bottle and monitored by the BACTEC 9240 instrument for 4 weeks. A total of 1,072 synovial fluid cultures were processed, and 15 (0.14%) were positive for Brucella melitensis. A single culture, harboring 1.3 CFU of viable organisms per ml, was missed by the instrument. All 14 positive BACTEC cultures were detected within 3 to 7 days.

* Corresponding author. Mailing address: Clinical Microbiology Laboratories, Soroka Medical Center, Ben-Gurion University of the Negev, Beer-Sheva, Israel 84101. Phone: (972)76400507. Fax: (972)76403541. E-mail: yagupsky{at}bgumail.bgu.ac.il.

Journal of Clinical Microbiology, February 2001, p. 738-739, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.738-739.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Durmaz, G., Us, T., Aydinli, A., Kiremitci, A., Kiraz, N., Akgun, Y. (2003). Optimum Detection Times for Bacteria and Yeast Species with the BACTEC 9120 Aerobic Blood Culture System: Evaluation for a 5-Year Period in a Turkish University Hospital. J. Clin. Microbiol. 41: 819-821 [Abstract] [Full Text]  


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