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Journal of Clinical Microbiology, February 2001, p. 769-771, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.769-771.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Sterilization of Mycobacterium tuberculosis Erdman Samples by Antimicrobial Fixation in a Biosafety Level 3 Laboratory

J. Reid Schwebach,1,* William R. Jacobs Jr.,1,2 and Arturo Casadevall1

Department of Microbiology and Immunology1 and The Howard Hughes Medical Institute,2 Albert Einstein College of Medicine, Bronx, New York 10461

Received 5 July 2000/Returned for modification 25 September 2000/Accepted 21 October 2000

Incomplete sterilization of Mycobacterium tuberculosis Erdman cultures followed 1 h of incubation in low concentrations of glutaraldehyde (0.5 and 1.0%) or azide. In contrast, 2.5% glutaraldehyde, paraformaldehyde (2 or 4%), Vesphine IIse or 5% formalin sterilized these samples after 1 h. These results suggest caution in removing fixed M. tuberculosis samples from biosafety level 3.

* Corresponding author. Mailing address: 703 Golding Building, 1300 Morris Park Ave., Bronx, NY 10461. Phone: (718) 430-3768. Fax: (718) 430-8701. E-mail: schwebac{at}aecom.yu.edu.

Journal of Clinical Microbiology, February 2001, p. 769-771, Vol. 39, No. 2
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.2.769-771.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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