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Journal of Clinical Microbiology, March 2001, p. 1073-1078, Vol. 39, No. 3
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.3.1073-1078.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Phenotypic Methods for Determining Genomovar Status of the Burkholderia cepacia Complex

Deborah A. Henry,1 Eshwar Mahenthiralingam,2 Peter Vandamme,3 Tom Coenye,3 and David P. Speert1,*

Department of Pediatrics, University of British Columbia, Vancouver, Canada1; School of Biosciences, Cardiff University, Cardiff, United Kingdom2; Microbiology Laboratory, Ghent University, Ghent, Belgium3

Received 19 September 2000/Returned for modification 28 November 2000/Accepted 1 January 2001

Recent taxonomic advances have demonstrated that Burkholderia cepacia is a cluster of at least seven closely related genomic species (or genomovars) collectively referred to as the B. cepacia complex, all of which may cause infections among cystic fibrosis patients and other vulnerable individuals. Thus, it is important for clinical microbiologists to be able to differentiate genomovars. Prior to this study, 361 B. cepacia complex isolates and 51 isolates easily confused with B. cepacia complex previously had been identified using a polyphasic approach, and in this study, a comparison of phenotypic and biochemical tests was carried out. It was determined that Burkholderia multivorans and Burkholderia stabilis could reliably be separated from other members of the B. cepacia complex by phenotypic methods. A combination of phenotypic and molecular tests such as recA PCR and 16S rRNA RFLP are recommended for differentiation among the genomovars of the B. cepacia complex. A biochemical reaction scheme for the identification of B. gladioli, Pandoraea species, and Ralstonia pickettii and the differentiation of these species from the B. cepacia complex is also presented.

* Corresponding author. Mailing address: British Columbia Research Institute for Children's and Women's Health, 950 W. 28th Ave., Room 375, Vancouver, BC, Canada V5Z 4H4. Phone: (604) 875-2438. Fax: (604) 875-2226. E-mail: speert{at}interchange.ubc.ca.

Journal of Clinical Microbiology, March 2001, p. 1073-1078, Vol. 39, No. 3
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.3.1073-1078.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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