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Journal of Clinical Microbiology, March 2001, p. 1114-1117, Vol. 39, No. 3
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.3.1114-1117.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Detection of Treponema denticola in Atherosclerotic Lesions

Katsuji Okuda,1,2,* Kazuyuki Ishihara,1,2 Taneaki Nakagawa,1,3 Akihiko Hirayama,1 Yoshiyuki Inayama,4 and Kenji Okuda5

Oral Health Science Center1 and Departments of Microbiology2 and Periodontics,3 Tokyo Dental College, Chiba, and Departments of Anatomic and Surgical Pathology4 and Bacteriology,5 Yokohama City University School of Medicine, Yokohama, Japan

Received 19 September 2000/Returned for modification 16 October 2000/Accepted 18 December 2000

We examined 26 atherosclerotic lesions and 14 nondiseased aorta specimens to detect the periodontopathogenic part of the bacterial 16S rRNA locus by PCR. Treponema denticola sequence of the 16S rRNA locus was found in 6 out of 26 DNA samples (23.1%) from the formalin-fixed, paraffin-embeded atherosclerotic lesions obtained during surgery but not in any of the 14 nondiseased aorta samples from deceased persons. Utilizing immunofluorescence microscopy, we observed aggregated antigenic particles reacting with rabbit antiserum against T. denticola in thin sections of the PCR-positive samples, but we could not detect any reacting particles in the PCR-negative thin sections.

* Corresponding author. Mailing address: Department of Microbiology and Oral Health Science Center, Tokyo Dental College, 1-2-2, Masago, Mihama-ku, Chiba, Japan. Phone: 81-43-270-3741. Fax: 81-43-270-3744. E-mail: kokuda{at}tdc.ac.jp.

Journal of Clinical Microbiology, March 2001, p. 1114-1117, Vol. 39, No. 3
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.3.1114-1117.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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