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Journal of Clinical Microbiology, March 2001, p. 1148-1151, Vol. 39, No. 3
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.3.1148-1151.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Comparison of Three Nucleic Acid Amplification Assays of Cerebrospinal Fluid for Diagnosis of Cytomegalovirus Encephalitis

Arabella Bestetti,1 Chiara Pierotti,1 Mariarosa Terreni,2 Alessandra Zappa,1 Luca Vago,3 Adriano Lazzarin,1 and Paola Cinque1,*

Division of Infectious Diseases, San Raffaele Hospital, 20127 Milan,1 Pathology Department, San Raffaele Hospital, 20132 Milan,2 and Pathology Department, L. Sacco Hospital, 20157 Milan,3 Italy

Received 21 August 2000/Returned for modification 4 October 2000/Accepted 1 December 2000

The diagnostic reliabilities of three cytomegalovirus (CMV) nucleic acid amplification assays of cerebrospinal fluid (CSF) were compared by using CSF samples from human immunodeficiency virus-infected patients with a postmortem histopathological diagnosis of CMV encephalitis (n = 15) or other central nervous system conditions (n = 16). By using a nested PCR assay, the quantitative COBAS AMPLICOR CMV MONITOR PCR, and the NucliSens CMV pp67 nucleic acid sequence-based amplification assay, sensitivities were 93.3, 86.6, and 93.3%, respectively, and specificities were 93.7, 93.7, and 87.5%, respectively. The COBAS AMPLICOR assay revealed significantly higher CMV DNA levels in patients with diffuse ventriculoencephalitis than in patients with focal periventricular lesions.

* Corresponding author. Mailing address: Division of Infectious Diseases, San Raffaele Hospital, Via Stamira d'Ancona 20, 20127 Milan, Italy. Phone: 39-02-26437985. Fax: 39-02-26437989. E-mail: cinque.paola{at}hsr.it.

Journal of Clinical Microbiology, March 2001, p. 1148-1151, Vol. 39, No. 3
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.3.1148-1151.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.




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Copyright © 2001 by the American Society for Microbiology. All rights reserved.