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Journal of Clinical Microbiology, March 2001, p. 1197-1199, Vol. 39, No. 3
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.3.1197-1199.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Evaluation of a Group-Specific 16S Ribosomal DNA-Based PCR for Detection of Helicobacter bizzozeronii, Helicobacter felis, and Helicobacter salomonis in Fresh and Paraffin-Embedded Gastric Biopsy Specimens

Dominic De Groote,1,* Freddy Haesebrouck,1 Leen-Jan van Doorn,2 Peter Vandamme,3 and Richard Ducatelle1

Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, B-9820 Merelbeke,1 and Laboratory of Microbiology, Faculty of Pharmaceutical Sciences, Ghent University, B-9000 Ghent,3 Belgium, and Delft Diagnostic Laboratory, 2600 GA Delft, The Netherlands2

Received 5 September 2000/Returned for modification 27 November 2000/Accepted 22 December 2000

A new specific and sensitive 16S ribosomal DNA-based PCR assay was developed. The assay targets a 78-bp DNA fragment unique to Helicobacter bizzozeronii, Helicobacter felis, and Helicobacter salomonis and can be used with freshly frozen and formalin-fixed paraffin-embedded gastric biopsy specimens.

* Corresponding author. Mailing address: Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Salisburylaan 133, B-9820 Merelbeke, Belgium. Phone: 32 9 264 77 45. Fax: 32 9 264 77 89. E-mail: dominic.degroote{at}rug.ac.be.

Journal of Clinical Microbiology, March 2001, p. 1197-1199, Vol. 39, No. 3
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.3.1197-1199.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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