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Journal of Clinical Microbiology, March 2001, p. 862-870, Vol. 39, No. 3
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.3.862-870.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Comparative Performance of Three Viral Load Assays on Human Immunodeficiency Virus Type 1 (HIV-1) Isolates Representing Group M (Subtypes A to G) and Group O: LCx HIV RNA Quantitative, AMPLICOR HIV-1 MONITOR Version 1.5, and Quantiplex HIV-1 RNA Version 3.0

Priscilla Swanson,1 Vincent Soriano,2 Sushil G. Devare,1 and John Hackett Jr.1,*

AIDS Research and Retrovirus Discovery, Abbott Laboratories, Abbott Park, Illinois 60064,1 and Service of Infectious Diseases, Instituto de Salud Carlos III, Madrid, Spain2

Received 12 September 2000/Accepted 29 November 2000

The performance of the LCx HIV RNA Quantitative (LCx HIV), AMPLICOR HIV-1 MONITOR version 1.5 (MONITOR v1.5), and Quantiplex HIV-1 RNA version 3.0 (bDNA v3.0) viral load assays was evaluated with 39 viral isolates (3 A, 7 B, 6 C, 4 D, 8 E, 4 F, 1 G, 4 mosaic, and 2 group O). Quantitation across the assay dynamic ranges was assessed using serial fivefold dilutions of the viruses. In addition, sequences of gag-encoded p24 (gag p24), pol-encoded integrase, and env-encoded gp41 were analyzed to assign group and subtype and to assess nucleotide mismatches at primer and probe binding sites. For group M isolates, quantification was highly correlated among all three assays. In contrast, only the LCx HIV assay reliably quantified group O isolates. The bDNA v3.0 assay detected but consistently underquantified group O viruses, whereas the MONITOR v1.5 test failed to detect group O viruses. Analysis of target regions revealed fewer primer or probe mismatches in the LCx HIV assay than in the MONITOR v1.5 test. Consistent with the high level of nucleotide conservation is the ability of the LCx HIV assay to quantify efficiently human immunodeficiency virus type 1 group M and the genetically diverse group O.

* Corresponding author. Mailing address: Abbott Laboratories, D-9NG, Bldg. AP20, 100 Abbott Park Rd., Abbott Park, IL 60064-6015. Phone: (847) 938-0457. Fax: (847) 937-1401. E-mail: john.hackett{at}add.ssw.abbott.com.

Journal of Clinical Microbiology, March 2001, p. 862-870, Vol. 39, No. 3
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.3.862-870.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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