This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shin, Y. K.
Right arrow Articles by Levin, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shin, Y. K.
Right arrow Articles by Levin, M. J.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, March 2001, p. 913-917, Vol. 39, No. 3
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.3.913-917.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Frequency of Acyclovir-Resistant Herpes Simplex Virus in Clinical Specimens and Laboratory Isolates

Young Kyoo Shin,1,2 Guang-Yun Cai,2 Adriana Weinberg,2 Jeffry J. Leary,3 and Myron J. Levin2,*

Department of Pediatrics, College of Medicine, Korea University, Seoul, Korea1; Section of Pediatric Infectious Diseases, School of Medicine, University of Colorado, Denver, Colorado2; and SmithKline Beecham Pharmaceuticals, Collegeville, Pennsylvania3

Received 7 August 2000/Returned for modification 6 November 2000/Accepted 22 February 2000

The proportion of acyclovir (ACV)-resistant herpes simplex virus (HSV) isolates in clinical specimens and laboratory isolates was determined. HSV isolates in clinical specimens and laboratory isolates were cultured in the absence or presence of 5 µg of ACV per ml. The frequency of ACV-resistant HSV was calculated as (average virus titer in the wells with ACV)/(average virus titer in the wells without ACV). The mutation frequency of HSV type 1 isolates in clinical samples (directly from patient lesions) was 7.5 × 10-4 ± 2.5 × 10-4 (mean ± standard error), and that of HSV type 2 isolates was 15.0 × 10-4 ± 4.6 × 10-4. The mutation frequencies of isolates derived in the laboratory from these clinical samples were very similar. Similarly, the 50% inhibitory concentrations for isolates in clinical samples and laboratory isolates were identical. The frequencies of ACV-resistant HSV types 1 and 2 were in a narrow range of 7.5 × 10-4 to 15.0 × 10-4 among isolates in clinical specimens and did not change for laboratory-derived pools of viral isolates.

* Corresponding author. Mailing address: University of Colorado Health Sciences Center, School of Medicine, 4200 East Ninth Ave., C-227, Denver, CO 80262. Phone: (303) 315-4620. Fax: (303) 315-7909. E-mail: myron.levin{at}uchsc.edu.

Journal of Clinical Microbiology, March 2001, p. 913-917, Vol. 39, No. 3
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.3.913-917.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Suzutani, T., Ishioka, K., De Clercq, E., Ishibashi, K., Kaneko, H., Kira, T., Hashimoto, K.-i., Ogasawara, M., Ohtani, K., Wakamiya, N., Saijo, M. (2003). Differential Mutation Patterns in Thymidine Kinase and DNA Polymerase Genes of Herpes Simplex Virus Type 1 Clones Passaged in the Presence of Acyclovir or Penciclovir. Antimicrob. Agents Chemother. 47: 1707-1713 [Abstract] [Full Text]  
  • Bacon, T. H., Levin, M. J., Leary, J. J., Sarisky, R. T., Sutton, D. (2003). Herpes Simplex Virus Resistance to Acyclovir and Penciclovir after Two Decades of Antiviral Therapy. Clin. Microbiol. Rev. 16: 114-128 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»