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Journal of Clinical Microbiology, April 2001, p. 1258-1263, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1258-1263.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Electrophoretic Karyotype Analysis of Sequential Candida parapsilosis Isolates from Patients with Persistent or Recurrent Fungemia

Jong Hee Shin,1,* Dong Hyeon Shin,2 Jeong Won Song,1 Seung Jung Kee,1 Soon Pal Suh,1 and Dong Wook Ryang1

Departments of Clinical Pathology1 and Internal Medicine,2 Chonnam National University Medical School, Kwangju, South Korea

Received 28 November 2000/Returned for modification 10 January 2001/Accepted 5 February 2001

We assessed the genetic relatedness of sequential isolates of Candida parapsilosis during persistent or recurrent fungemia and the effect of central venous catheter (CVC) removal. Serial isolates of C. parapsilosis were obtained from 17 patients with persistent or recurrent fungemia over periods of up to 5 months. Forty-eight C. parapsilosis isolates from the blood of 17 patients were analyzed by electrophoretic karyotyping (EK) with pulsed-field gel electrophoresis (PFGE), revealing 25 different karyotypes. The strains sequentially isolated from each of seven patients whose fungemia resolved following CVC removal had the same karyotype. Two patients with fungemia that cleared without CVC removal each had two sequential isolates with different karyotypes. In six (75%) of the eight patients whose fungemia was recurrent even after CVC removal, the karyotypes of the pre- and post-CVC removal isolates were different, implying the emergence of a new strain. Overall, the sequential strains from each patient had identical karyotypes in 53% (9 of 17) of the patients and two different karyotypes in 47% (8 of 17). This study shows that EK with PFGE is useful for investigating persistent or recurrent fungemia due to C. parapsilosis and that recurrent fungemia due to C. parapsilosis is more likely caused by reinfection with a second strain.

* Corresponding author. Mailing address: Department of Clinical Pathology, Chonnam National University Medical School, 8 Hakdong Dongku, Kwangju 501-757, South Korea. Phone: 82 (62) 220-5342. Fax: 82 (62) 224-2518. E-mail: shinjh{at}chonnam.ac.kr.

Journal of Clinical Microbiology, April 2001, p. 1258-1263, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1258-1263.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Shin, J. H., Chae, M. J., Song, J. W., Jung, S.-I., Cho, D., Kee, S. J., Kim, S. H., Shin, M. G., Suh, S. P., Ryang, D. W. (2007). Changes in Karyotype and Azole Susceptibility of Sequential Bloodstream Isolates from Patients with Candida glabrata Candidemia. J. Clin. Microbiol. 45: 2385-2391 [Abstract] [Full Text]  
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