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Journal of Clinical Microbiology, April 2001, p. 1345-1347, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1345-1347.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Inoculum Standardization for Antifungal Susceptibility Testing of Filamentous Fungi Pathogenic for Humans

Eva Petrikkou, Juan L. Rodríguez-Tudela,* Manuel Cuenca-Estrella, Alicia Gómez, Ana Molleja, and Emilia Mellado

Servicio de Micologia, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, 28220 Majadahonda, Spain

Received 16 October 2000/Returned for modification 27 December 2000/Accepted 26 January 2001

Two methods of inoculum preparation for filamentous fungi were compared: counting with a hematocytometer and spectrophotometric adjustment. One hundred eighty-two filamentous fungi pathogenic for humans were used. Colony counts were done for all inoculum preparations. The agreement between the hematocytometer counts and the colony counts (CFU per milliliter) was 97.2%. The reproducibility between the hematocytometer counts and the colony counts by means of an intraclass correlation coefficient was 0.70. Pearson's correlation index for hematocytometer counts versus colony counts was 0.56, whereas that for optical density versus colony counts was 0.008. Both methods can be used for inoculum size adjustment. However, the use of the spectrophotometric method requires that each species be standardized separately.

* Corresponding author. Mailing address: Servicio de Micología, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Ctra. Majadahonda-Pozuelo km. 2, 28220 Majadahonda, Spain. Phone: 34 91 5097961. Fax: 34 91 5097966. E-mail: juanl.rodriguez-tudela{at}isciii.es.

Journal of Clinical Microbiology, April 2001, p. 1345-1347, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1345-1347.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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