This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bas, S.
Right arrow Articles by Vischer, T. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bas, S.
Right arrow Articles by Vischer, T. L.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, April 2001, p. 1368-1377, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1368-1377.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Chlamydial Serology: Comparative Diagnostic Value of Immunoblotting, Microimmunofluorescence Test, and Immunoassays Using Different Recombinant Proteins as Antigens

S. Bas,1,* P. Muzzin,2 B. Ninet,3 J. E. Bornand,4 C. Scieux,5 and T. L. Vischer1

Division of Rheumatology,1 Division of Infectious Diseases,3 and Virology Laboratory, Department of Internal Medicine,4 University Hospital, and Medical Biochemistry Department, Geneva Medical School,2 1211 Geneva 14, Switzerland, and Bacteriology Laboratory, Saint-Louis Hospital, 75475 Paris, France5

Received 7 September 2000/Returned for modification 23 October 2000/Accepted 3 January 2001

To improve the reliability of the serodiagnosis of Chlamydia trachomatis infections, an immunoblot analysis, a microimmunofluorescence titration, and different immunoassays using synthetic peptides derived from species-specific epitopes in variable domain IV of the major outer membrane protein or recombinant antigens (heat shock protein 70 [hsp70], hsp60, hsp10, polypeptide encoded by open reading frame 3 of the plasmid [pgp3], macrophage infectivity potentiator, and a fragment of the total lipopolysaccharide) were evaluated. Because cross-reactions between chlamydial species have been reported, the microimmunofluorescence tests were also performed with Chlamydia pneumoniae and Chlamydia psittaci used as antigens, and C. pneumoniae-specific antibodies were also determined by immunoassays. Since the presence of antimicrobial antibodies must be interpreted in light of their prevalence in the general population, responses obtained with serum samples from patients with well-defined infection (i.e., with positive urethral or endocervical C. trachomatis DNA amplification) were compared to those obtained with samples from healthy blood donors. The best sensitivity (86%) with a specificity of 81% was obtained for immunoblotting results, when the number of individuals with >= 10 immunoglobulin G (IgG) and/or >= 2 IgM responses to the different C. trachomatis antigens was considered. A 13-kDa antigen was recognized by most of the samples (86% for IgG) from patients with acute urogenital infection but rarely (3%) by those from healthy blood donors (P < 0.0001). The sensitivity and specificity results obtained for serum antibodies to peptides or recombinant antigens were slightly lower than those results obtained for the number of responses to whole C. trachomatis antigens, which were 76 and 77%, respectively, when IgG responses to both recombinant hsp60 and pgp3 were considered.

* Corresponding author. Mailing address: Division of Rheumatology, Department of Internal Medicine, University Hospital, 1211 Geneva 14, Switzerland. Phone: (41 22) 382 36 80. Fax: (41 22) 382 35 30. E-mail: bas-sylvette{at}diogenes.hcuge.ch.

Journal of Clinical Microbiology, April 2001, p. 1368-1377, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1368-1377.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Wills, G. S., Horner, P. J., Reynolds, R., Johnson, A. M., Muir, D. A., Brown, D. W., Winston, A., Broadbent, A. J., Parker, D., McClure, M. O. (2009). Pgp3 Antibody Enzyme-Linked Immunosorbent Assay, a Sensitive and Specific Assay for Seroepidemiological Analysis of Chlamydia trachomatis Infection. CVI 16: 835-843 [Abstract] [Full Text]  
  • Gorander, S., Lagergard, T., Romanik, M., Viscidi, R. P., Martirosian, G., Liljeqvist, J.-A. (2008). Seroprevalences of Herpes Simplex Virus Type 2, Five Oncogenic Human Papillomaviruses, and Chlamydia trachomatis in Katowice, Poland. CVI 15: 675-680 [Abstract] [Full Text]  
  • Bas, S., Neff, L., Vuillet, M., Spenato, U., Seya, T., Matsumoto, M., Gabay, C. (2008). The Proinflammatory Cytokine Response to Chlamydia trachomatis Elementary Bodies in Human Macrophages Is Partly Mediated by a Lipoprotein, the Macrophage Infectivity Potentiator, through TLR2/TLR1/TLR6 and CD14. J. Immunol. 180: 1158-1168 [Abstract] [Full Text]  
  • Sutcliffe, S., Giovannucci, E., Gaydos, C. A., Viscidi, R. P., Jenkins, F. J., Zenilman, J. M., Jacobson, L. P., De Marzo, A. M., Willett, W. C., Platz, E. A. (2007). Plasma Antibodies against Chlamydia trachomatis, Human Papillomavirus, and Human Herpesvirus Type 8 in Relation to Prostate Cancer: A Prospective Study. Cancer Epidemiol. Biomarkers Prev. 16: 1573-1580 [Abstract] [Full Text]  
  • Neff, L., Daher, S., Muzzin, P., Spenato, U., Gulacar, F., Gabay, C., Bas, S. (2007). Molecular Characterization and Subcellular Localization of Macrophage Infectivity Potentiator, a Chlamydia trachomatis Lipoprotein. J. Bacteriol. 189: 4739-4748 [Abstract] [Full Text]  
  • Colmegna, I., Cuchacovich, R., Espinoza, L. R. (2004). HLA-B27-Associated Reactive Arthritis: Pathogenetic and Clinical Considerations. Clin. Microbiol. Rev. 17: 348-369 [Abstract] [Full Text]  
  • Land, J.A., Gijsen, A.P., Kessels, A.G.H., Slobbe, M.E.P., Bruggeman, C.A. (2003). Performance of five serological chlamydia antibody tests in subfertile women. Hum Reprod 18: 2621-2627 [Abstract] [Full Text]  
  • Bas, S., Genevay, S., Schenkel, M.-C., Vischer, T. L. (2002). Importance of species-specific antigens in the serodiagnosis of Chlamydia trachomatis reactive arthritis. Rheumatology (Oxford) 41: 1017-1020 [Abstract] [Full Text]  
  • Witkin, S. S., Linhares, I. M. (2002). Chlamydia trachomatis in subfertile women undergoing uterine instrumentation: An alternative to direct microbial testing or prophylactic antibiotic treatment. Hum Reprod 17: 1938-1941 [Abstract] [Full Text]  
  • Chernesky, M A (2002). Chlamydia trachomatis diagnostics. Sex. Transm. Infect. 78: 232-234 [Abstract] [Full Text]  
  • Marston, E. L., James, A. V., Parker, J. T., Hart, J. C., Brown, T. M., Messmer, T. O., Jue, D. L., Black, C. M., Carlone, G. M., Ades, E. W., Sampson, J. (2002). Newly Characterized Species-Specific Immunogenic Chlamydophila pneumoniae Peptide Reactive with Murine Monoclonal and Human Serum Antibodies. CVI 9: 446-452 [Abstract] [Full Text]  
  • Bas, S., Muzzin, P., Vischer, T. L. (2001). Chlamydia trachomatis Serology: Diagnostic Value of Outer Membrane Protein 2 Compared with That of Other Antigens. J. Clin. Microbiol. 39: 4082-4085 [Abstract] [Full Text]  
  • Stralin, K., Fredlund, H., Olcen, P., Bas, S., Vischer, T. L. (2001). Labsystems Enzyme Immunoassay for Chlamydia pneumoniae Also Detects Chlamydia psittaci Infections. J. Clin. Microbiol. 39: 3425-3426 [Full Text]  
  • Bas, S., Muzzin, P., Fulpius, T., Buchs, N., Vischer, T. L. (2001). Indirect evidence of intra-articular immunoglobulin G synthesis in patients with Chlamydia trachomatis reactive arthritis. Rheumatology (Oxford) 40: 801-805 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»