This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Phuektes, P.
Right arrow Articles by Browning, G. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Phuektes, P.
Right arrow Articles by Browning, G. F.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, April 2001, p. 1460-1466, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1460-1466.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Molecular Epidemiology of Streptococcus uberis Isolates from Dairy Cows with Mastitis

Patchara Phuektes,1 Peter D. Mansell,2 Rodney S. Dyson,3 Narelle D. Hooper,3 Jennifer S. Dick,3 and Glenn F. Browning1,*

Veterinary Preclinical Centre, Faculty of Veterinary Science, The University of Melbourne, Melbourne,1 Veterinary Clinical Centre, Faculty of Veterinary Science, The University of Melbourne, Werribee,2 and Kyabram Veterinary Clinic, Kyabram,3 Victoria, Australia

Received 21 June 2000/Returned for modification 11 October 2000/Accepted 29 January 2001

Pulsed-field gel electrophoresis and antimicrobial sensitivity testing were used as tools to investigate the epidemiology of Streptococcus uberis mastitis in dairy cows. A total of 62 different strains were found among 138 isolates from the four herds investigated, and between 10 and 26 different strains were found in each herd. There was no strain common to all four herds. Identical strains of S. uberis were detected from different quarters of individual cows and from cows within the same herd, suggesting that transmission from quarter to quarter and cow to cow had occurred. Despite the great variation in S. uberis strains, persistent infection with the same strain within a lactation was observed in most cows. Predominant strains were present in two herds. Preliminary investigations could not clarify why these particular strains might predominate, but in one herd there was a significant difference between the prevalence of clinical mastitis in quarters infected with the predominant strain and that in quarters infected with other strains, suggesting the greater virulence of the predominant strain. The wide variety of S. uberis strains found is consistent with an environmental source of S. uberis. However, evidence of direct transmission, the persistence of infection, and the predominance of particular strains in some herds indicate that S. uberis infections are epidemiologically complex and that the relative importance of these factors in the occurrence of mastitis may differ between herds.

* Corresponding author. Mailing address: Veterinary Preclinical Centre, Faculty of Veterinary Science, The University of Melbourne, Melbourne, Victoria 3010, Australia. Phone: (613) 8344 7342. Fax: (613) 8344 7374. E-mail: glenfb{at}unimelb.edu.au.

Journal of Clinical Microbiology, April 2001, p. 1460-1466, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1460-1466.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Pryor, S. M., Cursons, R. T., Williamson, J. H., Lacy-Hulbert, S. J. (2009). Experimentally induced intramammary infection with multiple strains of Streptococcus uberis. J DAIRY SCI 92: 5467-5475 [Abstract] [Full Text]  
  • Nam, H.-m., Lim, S.-K., Kang, H.-M., Kim, J.-M., Moon, J.-S., Jang, K.-C., Joo, Y.-S., Kang, M.-i., Jung, S.-C. (2009). Antimicrobial resistance of streptococci isolated from mastitic bovine milk samples in Korea. jvdi 21: 698-701 [Abstract] [Full Text]  
  • Rato, M. G., Bexiga, R., Nunes, S. F., Cavaco, L. M., Vilela, C. L., Santos-Sanches, I. (2008). Molecular Epidemiology and Population Structure of Bovine Streptococcus uberis. J DAIRY SCI 91: 4542-4551 [Abstract] [Full Text]  
  • Tomita, T., Meehan, B., Wongkattiya, N., Malmo, J., Pullinger, G., Leigh, J., Deighton, M. (2008). Identification of Streptococcus uberis Multilocus Sequence Types Highly Associated with Mastitis. Appl. Environ. Microbiol. 74: 114-124 [Abstract] [Full Text]  
  • Coffey, T. J., Pullinger, G. D., Urwin, R., Jolley, K. A., Wilson, S. M., Maiden, M. C., Leigh, J. A. (2006). First Insights into the Evolution of Streptococcus uberis: a Multilocus Sequence Typing Scheme That Enables Investigation of Its Population Biology. Appl. Environ. Microbiol. 72: 1420-1428 [Abstract] [Full Text]  
  • Pullinger, G. D., Lopez-Benavides, M., Coffey, T. J., Williamson, J. H., Cursons, R. T., Summers, E., Lacy-Hulbert, J., Maiden, M. C., Leigh, J. A. (2006). Application of Streptococcus uberis Multilocus Sequence Typing: Analysis of the Population Structure Detected among Environmental and Bovine Isolates from New Zealand and the United Kingdom. Appl. Environ. Microbiol. 72: 1429-1436 [Abstract] [Full Text]  
  • Zadoks, R. N., Schukken, Y. H., Wiedmann, M. (2005). Multilocus Sequence Typing of Streptococcus uberis Provides Sensitive and Epidemiologically Relevant Subtype Information and Reveals Positive Selection in the Virulence Gene pauA. J. Clin. Microbiol. 43: 2407-2417 [Abstract] [Full Text]  
  • McDougall, S., Parkinson, T. J., Leyland, M., Anniss, F. M., Fenwick, S. G. (2004). Duration of Infection and Strain Variation in Streptococcus uberis Isolated from Cows' Milk. J DAIRY SCI 87: 2062-2072 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»