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Journal of Clinical Microbiology, April 2001, p. 1510-1516, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1510-1516.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Specific Detection and Prevalence of Helicobacter heilmannii-Like Organisms in the Human Gastric Mucosa by Fluorescent In Situ Hybridization and Partial 16S Ribosomal DNA Sequencing

K. Trebesius,1 K. Adler,1 M. Vieth,2 M. Stolte,2 and R. Haas1,*

Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians University, Munich,1 and Institute for Pathology, Klinikum, Bayreuth,2 Germany

Received 11 September 2000/Returned for modification 28 October 2000/Accepted 27 January 2001

Gastric infection with Helicobacter heilmannii (previously known as Gastrospirillum hominis) is invariably linked with the presence of chronic gastritis and the risk of developing low-grade mucosa-associated lymphoid tissue lymphoma in humans. In contrast to Helicobacter pylori, various H. heilmannii species colonize the stomachs of domestic animals, which might be a reservoir for transmission to humans (zoonosis). To identify the number and prevalence of different H. heilmanni types in humans, we analyzed 89 gastric biopsy samples histologically identified as H. heilmannii positive by fluorescence in situ hybridization. Of these gastric specimens, 84 (94.4%) contained a single H. heilmannii type. In five samples, however, two different H. heilmannii types were detected. The most prevalent species in monoinfected samples is H. heilmannii type 1, found in 78.5% (66 of 84) of the specimens, followed by a novel H. heilmannii-like organism (HHLO), HHLO type 4, identified in 9.6% (8 of 84) of tissue sections. H. heilmannii type 2 and a further HHLO type not described before, type 3, were found in 8.3% (7 of 84) and 1.2% (1 of 84) of the monoinfected samples, respectively. Additionally, HHLO type 5 with a 16S ribosomal DNA sequence identical to that of Helicobacter salomonis was found with a prevalence of 2.4% (2 of 89). Thirteen of these biopsy samples were also investigated by a PCR approach developed for this study that allows a Helicobacter-specific amplification of a variable portion of the 16S rRNA gene and subsequent sequencing. In total, five different types of HHLOs could be identified within these samples. We conclude that humans can be infected by at least five different HHLO types, which presumably have their origin in animal species like dogs, cats, and pigs.

* Corresponding author. Mailing address: Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Pettenkoferstr. 9a, D-80336 Munich, Germany. Phone: 49-89-5160 5255. Fax: 49-89-5160 5223. E-mail: haas{at}m3401.mpk.med.uni-muenchen.de.

Journal of Clinical Microbiology, April 2001, p. 1510-1516, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1510-1516.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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