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Journal of Clinical Microbiology, April 2001, p. 1600-1603, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1600-1603.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Typing of Candida albicans Isolates by Sequence Analysis of the Cytochrome b Gene and Differentiation from Candida stellatoidea

Swarajit Kumar Biswas, Koji Yokoyama,* Li Wang, Kazuko Nishimura, and Makoto Miyaji

Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8673, Japan

Received 11 December 2000/Returned for modification 8 January 2001/Accepted 29 January 2001

Including type strains, mitochondrial cytochrome b genes of 32 strains of Candida albicans and 6 strains of Candida stellatoidea, presently treated as a synonym for C. albicans, were partially sequenced. Analysis of 396-bp nucleotide sequences of the strains under investigation divided C. albicans isolates into three types: type I, type II, and type III; however, strains of C. stellatoidea represented distinct type IV isolates. Deduced amino acid sequences of type I, type II, and type III were identical and differed from that of type IV by one amino acid. Genotypes (rDNA type) of the test strains were also checked. Cytochrome b typing did not correlate with genotyping, and different genotypes occurred for one cytochrome b type. This study shows that cytochrome b gene sequences are useful for analyzing the genetic relatedness of C. albicans isolates and effective for differentiating C. stellatoidea from C. albicans.

* Corresponding author. Mailing address: Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8673, Japan. Phone: 81-43-226-2789. Fax: 81-43-226-2486. E-mail: yoko{at}myco.pf.chiba-u.ac.jp.

Journal of Clinical Microbiology, April 2001, p. 1600-1603, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1600-1603.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Biswas, S. K., Wang, L., Yokoyama, K., Nishimura, K. (2005). Molecular Phylogenetics of the Genus Trichosporon Inferred from Mitochondrial Cytochrome b Gene Sequences. J. Clin. Microbiol. 43: 5171-5178 [Abstract] [Full Text]  
  • Robles, J. C., Koreen, L., Park, S., Perlin, D. S. (2004). Multilocus Sequence Typing Is a Reliable Alternative Method to DNA Fingerprinting for Discriminating among Strains of Candida albicans. J. Clin. Microbiol. 42: 2480-2488 [Abstract] [Full Text]  
  • Biswas, S. K., Wang, L., Yokoyama, K., Nishimura, K. (2003). Molecular Analysis of Cryptococcus neoformans Mitochondrial Cytochrome b Gene Sequences. J. Clin. Microbiol. 41: 5572-5576 [Abstract] [Full Text]  
  • Chong, P. P., Lee, Y. L., Tan, B. C., Ng, K. P. (2003). Genetic relatedness of Candida strains isolated from women with vaginal candidiasis in Malaysia. J Med Microbiol 52: 657-666 [Abstract] [Full Text]  


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