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Journal of Clinical Microbiology, April 2001, p. 1615-1617, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1615-1617.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Fluconazole Disk Diffusion Test with Methylene Blue- and Glucose-Enriched Mueller-Hinton Agar for Determining Susceptibility of Candida Species

Sai-Cheong Lee,1,* Chang-Phone Fung,2 Ning Lee,3 Lai-Chu See,4 Jen-Seng Huang,5 Chi-Jen Tsai,5 Kuo-Su Chen,5 and Wen-Ben Shieh5

Division of Infectious Diseases1 and Departments of Pathology3 and Internal Medicine,5 Chang Gung Memorial Hospital, Keelung, Department of Public Health, Chang Gung University, Linkou,4 and Division of Infectious Diseases, Veterans General Hospital, Taipei,2 Taiwan, Republic of China

Received 5 September 2000/Returned for modification 5 November 2000/Accepted 10 January 2001

A 25-µg fluconazole disk diffusion test using a Mueller-Hinton agar plate containing 2% glucose and 5 µg of methylene blue/ml (GM-MH) was compared to the macrodilution reference method for 210 Candida species. The GM-MH agar plate was read at 24 h. The predictive values of disks with susceptible, intermediate, and resistant results on the GM-MH agar plate at 24 h were 97.1, 56.3, and 76.5%, respectively.

* Corresponding author. Mailing address: Division of Infectious Diseases, Chang Gung Memorial Hospital, 222, Mai Chin Rd., Keelung, Taiwan, Republic of China. Phone: 886-02-24313131. Fax: 886-02-24335342. E-mail: Ruby800{at}adm.cgmh.com.tw.

Journal of Clinical Microbiology, April 2001, p. 1615-1617, Vol. 39, No. 4
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.4.1615-1617.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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