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Journal of Clinical Microbiology, May 2001, p. 1808-1812, Vol. 39, No. 5
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.5.1808-1812.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Sensitivity and Specificity of Human Immunodeficiency Virus Rapid Serologic Assays and Testing Algorithms in an Antenatal Clinic in Abidjan, Ivory Coast

Stéphania Koblavi-Dème,1 Chantal Maurice,1 Daniel Yavo,1 Toussaint S. Sibailly,1 Kabran N'guessan,1 Yvonne Kamelan-Tano,1 Stefan Z. Wiktor,1,2 Thierry H. Roels,1,2 Terence Chorba,1,2 and John N. Nkengasong1,2,*

Projet RETRO-CI, CHU de Treichville, Abidjan, Ivory Coast,1 and Division of HIV/AIDS Prevention, National Center for HIV, STD, and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia2

Received 13 November 2000/Returned for modification 29 January 2001/Accepted 28 February 2001

To evaluate serologic testing algorithms for human immunodeficiency virus (HIV) based on a combination of rapid assays among persons with HIV-1 (non-B subtypes) infection, HIV-2 infection, and HIV-1-HIV-2 dual infections in Abidjan, Ivory Coast, a total of 1,216 sera with known HIV serologic status were used to evaluate the sensitivity and specificity of four rapid assays: Determine HIV-1/2, Capillus HIV-1/HIV-2, HIV-SPOT, and Genie II HIV-1/HIV-2. Two serum panels obtained from patients recently infected with HIV-1 subtypes B and non-B were also included. Based on sensitivity and specificity, three of the four rapid assays were evaluated prospectively in parallel (serum samples tested by two simultaneous rapid assays) and serial (serum samples tested by two consecutive rapid assays) testing algorithms. All assays were 100% sensitive, and specificities ranged from 99.4 to 100%. In the prospective evaluation, both the parallel and serial algorithms were 100% sensitive and specific. Our results suggest that rapid assays have high sensitivity and specificity and, when used in parallel or serial testing algorithms, yield results similar to those of enzyme-linked immunosorbent assay-based testing strategies. HIV serodiagnosis based on rapid assays may be a valuable alternative in implementing HIV prevention and surveillance programs in areas where sophisticated laboratories are difficult to establish.

* Corresponding author: Mailing address: Laboratory of Virology, Projet RETRO-CI, 01 BP 1712, Abidjan 01, Ivory Coast. Phone: 225-21 25 41 89. Fax: 225-21 24 29 69. E-mail: jcn5{at}cdc.gov.

Journal of Clinical Microbiology, May 2001, p. 1808-1812, Vol. 39, No. 5
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.5.1808-1812.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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