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Journal of Clinical Microbiology, May 2001, p. 1993-1995, Vol. 39, No. 5
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.5.1993-1995.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Use of the Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test for Early Detection of Mycobacterium tuberculosis in BACTEC 12B Medium

E. P. Desmond* and K. Loretzdagger

Microbial Diseases Laboratory, Division of Communicable Disease Control, California Department of Health Services, Berkeley, California 94704

Received 11 December 2000/Returned for modification 3 February 2001/Accepted 4 March 2001

To achieve better sensitivity than direct testing and better turnaround time than current culture and identification methods, the Gen-Probe Mycobacterium Tuberculosis Direct method was used to detect Mycobacterium tuberculosis in BACTEC 12B medium cultures when they first gave a growth index (GI) of at least 10 (MTD/BACTEC method). Of 179 acid-fast, smear-positive specimens that were culture positive for M. tuberculosis, all were positive by the MTD/BACTEC method (sensitivity, 100%). Positive results were obtained only with tuberculosis patients. For diagnostic specimens from untreated patients, the mean time to achieve a GI of 10 was 6 days.

* Corresponding author. Mailing address: Microbial Diseases Laboratory, California Department of Health Services, 2151 Berkeley Way, Berkeley, CA 94704. Phone: (510) 540-2074. Fax: (510) 540-2374. E-mail: edesmond{at}dhs.ca.gov.

dagger Present address: School of Public Health, University of California, Berkeley, CA 94720.

Journal of Clinical Microbiology, May 2001, p. 1993-1995, Vol. 39, No. 5
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.5.1993-1995.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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