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Journal of Clinical Microbiology, May 2001, p. 2025-2027, Vol. 39, No. 5
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.5.2025-2027.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Endocarditis Caused by Culture-Negative Organisms Visible by Brown and Brenn Staining: Utility of PCR and DNA Sequencing for Diagnosis

Marissa B. Wilck,1 Yanyun Wu,1 John G. Howe,1 Jill Y. Crouch,1 and Stephen C. Edberg1,2,*

Departments of Laboratory Medicine1 and Internal Medicine,2 Yale University School of Medicine, New Haven, Connecticut

Received 18 September 2000/Returned for modification 25 November 2000/Accepted 28 February 2001

Two cases of culture-negative endocarditis with cocci seen in valve vegetations are presented. The organisms were identified by molecular analysis using broad-range PCR primers complementary to the 16S rRNA gene, sequencing, and database search using BLAST software. The results and utility of this method are discussed.

* Corresponding author. Mailing address: Laboratory Medicine, Yale University School of Medicine, P.O. Box 208035, New Haven, CT 06504. Phone: (203) 688-2457. Fax: (203) 688-4170. E-mail: stephen.edberg{at}yale.edu.

Journal of Clinical Microbiology, May 2001, p. 2025-2027, Vol. 39, No. 5
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.5.2025-2027.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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