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Journal of Clinical Microbiology, June 2001, p. 2280-2282, Vol. 39, No. 6
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.6.2280-2282.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Evaluation of the Roche Neisseria gonorrhoeae 16S rRNA PCR for Confirmation of AMPLICOR PCR-Positive Samples and Comparison of Its Diagnostic Performance According to Storage Conditions and Preparation of Endocervical Specimens

E. Van Dyck,* H. Smet, L. Van Damme, and M. Laga

Department of Microbiology, Institute of Tropical Medicine, Antwerp, Belgium

Received 22 November 2000/Returned for modification 6 February 2001/Accepted 21 March 2001

The AMPLICOR PCR was used to detect Neisseria gonorrhoeae in endocervical specimens. A 16S rRNA PCR performed on N. gonorrhoeae-positive samples showed sensitivities of 73.2, 64.3, and 94.4% for samples treated directly with AMPLICOR lysis buffer, samples suspended in 2-sucrose phosphate, and samples suspended in diluted phosphate-buffered saline, respectively.

* Corresponding author. Mailing address: STD/HIV Research and Intervention Unit, Department of Microbiology, Institute of Tropical Medicine, Nationalestraat 155, B-2000 Antwerp, Belgium. Phone: 32 3 247 63 29. Fax: 32 3 247 63 33. E-mail: evandyck{at}itg.be.

Journal of Clinical Microbiology, June 2001, p. 2280-2282, Vol. 39, No. 6
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.6.2280-2282.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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