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Journal of Clinical Microbiology, June 2001, p. 2308-2310, Vol. 39, No. 6
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.6.2308-2310.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Development of Reverse Transcriptase PCR Assays for Detection of Active Human Herpesvirus 6 Infection

Glenn Van den Bosch,1 Giuseppe Locatelli,2 Lieve Geerts,1 Giovanni Fagà,2 Margareta Ieven,3 Herman Goossens,3 Disa Bottiger,4 Bo Öberg,4,5 Paolo Lusso,2 and Zwi N. Berneman1,*

Laboratory of Experimental Hematology1 and Laboratory of Medical Microbiology3, University of Antwerp, Antwerp University Hospital, B-2650 Edegem, Belgium; Human Virology Unit, DIBIT, San Raffaele Research Institute, 20132 Milan, Italy2; and Medivir AB, S-141 44 Huddinge,4 and Microbiology and Tumor Biology Centre, Karolinska Institute, S-171 77 Stockholm5, Sweden

Received 25 August 2000/Returned for modification 6 October 2000/Accepted 20 March 2001

We developed reverse transcriptase (RT) PCR assays for the detection of mRNA from three spliced genes of human herpesvirus 6 (HHV-6), the immediate-early genes U16/U17 and U89/U90 and the late gene U60/U66. Sequence analysis determined the splicing sites of these genes. The new assays may be instrumental in investigating the association between HHV-6 and disease.

* Corresponding author. Mailing address: Division of Hematology, University of Antwerp, Antwerp University Hospital, Wilrijkstraat 10, B-2650 Edegem, Belgium. Phone: 32-3-821-3780. Fax: 32-3-821-4286. E-mail: zwi.berneman{at}uza.uia.ac.be.

Journal of Clinical Microbiology, June 2001, p. 2308-2310, Vol. 39, No. 6
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.6.2308-2310.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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