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Journal of Clinical Microbiology, June 2001, p. 2317-2320, Vol. 39, No. 6
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.6.2317-2320.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Rapid Receptor-Clustering Assay To Detect Uropathogenic and Diarrheal Escherichia coli Isolates Bearing Adhesins of the Dr Family

P. Goluszko,* R. Selvarangan,dagger B. J. Nowicki, S. Nowicki, A. Hart, E. Pawelczyk, and K. Nguyen

Department of Obstetrics & Gynecology, The University of Texas Medical Branch, Galveston, Texas 77555-1062

Received 19 October 2000/Returned for modification 2 January 2001/Accepted 11 March 2001

Infections caused by Escherichia coli isolates expressing adhesins of the Dr family are associated with diarrhea and urinary tract infections, and these E. coli strains recognize the complement regulatory protein decay-accelerating factor (DAF) as their receptor. Clustering of the DAF receptor at the sites of bacterial adherence to epithelial cells is proposed as an alternative to PCR assay for rapid detection of Dr-positive E. coli.

* Corresponding author. Mailing address: Department of Obstetrics & Gynecology, Division of Infectious Diseases, University of Texas Medical Branch, Galveston, TX 77555. Phone: (409) 772-2221. Fax: (409) 747-0475. E-mail: pgoluszk{at}utmb.edu.

dagger Present address: Division of Clinical Microbiology, Department of Laboratory Medicine, University of Washington, Seattle, WA 98195-7110.

Journal of Clinical Microbiology, June 2001, p. 2317-2320, Vol. 39, No. 6
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.6.2317-2320.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Das, M., Hart-Van Tassell, A., Urvil, P. T., Lea, S., Pettigrew, D., Anderson, K. L., Samet, A., Kur, J., Matthews, S., Nowicki, S., Popov, V., Goluszko, P., Nowicki, B. J. (2005). Hydrophilic Domain II of Escherichia coli Dr Fimbriae Facilitates Cell Invasion. Infect. Immun. 73: 6119-6126 [Abstract] [Full Text]  
  • Servin, A. L. (2005). Pathogenesis of Afa/Dr Diffusely Adhering Escherichia coli. Clin. Microbiol. Rev. 18: 264-292 [Abstract] [Full Text]  


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