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Journal of Clinical Microbiology, July 2001, p. 2581-2583, Vol. 39, No. 7
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.7.2581-2583.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Performance of the Chromogenic Medium CHROMagar Staph Aureus and the Staphychrom Coagulase Test in the Detection and Identification of Staphylococcus aureus in Clinical Specimens

Anne Carricajo,1 Axel Treny,2 Nathalie Fonsale,1 Michele Bes,3 Marie Elisabeth Reverdy,3 Yves Gille,2 Gerald Aubert,1 and Anne Marie Freydiere2,*

Hôpital de Bellevue, F-42055 Saint Etienne,1 Hôpital de l'Antiquaille, F-69321 Lyon cedex 5,2 and Hôpital Edouard Herriot, F-69437 Lyon cedex 03,3 France

Received 12 March 2001/Returned for modification 13 April 2001/Accepted 7 May 2001

CHROMagar Staph aureus (CSAM) (CHROMagar Microbiology, Paris, France) is a new chromogenic medium designed to enable detection of colonies of Staphylococcus aureus by their pink color. A total of 775 specimens were cultured in parallel on CHROMagar Staph aureus and conventional media. Among the 267 S. aureus strains recovered on at least one medium, 263 were isolated on CSAM medium (sensitivity, 98.5%), and 245 (sensitivity, 91.8%) were isolated on conventional media. The specificity of presumptive identification of S. aureus on the basis of pink colony color on CSAM medium was 97% (493 of 508). This specificity increased to 100% when coagulase detection with the Staphychrom coagulase test was added and to 98.8% when S. aureus surface components were detected by agglutination in the Pastorex Staph Plus test. Susceptibility testing of 67 S. aureus strains, performed in parallel on pink CSAM colonies and on colonies grown on blood agar, gave similar results. Thus, rapid and accurate recognition and identification of S. aureus isolates were achieved with CSAM as the primary isolation medium, followed by the staphylocoagulase Staphychrom test. Antimicrobial susceptibility testing (disk-diffusion method or ATB STAPH System) can be performed directly on pink CSAM colonies.

* Corresponding author. Present address: Laboratoire de microbiologie, Hôpital Debrousse, 29 rue Soeur Bouvier, 69322 Lyon cedex 05, France. Phone: 33 4 72 38 58 16. Fax: 33 4 72 38 55 35. E-mail: am.freydiere{at}chu-lyon.fr.

Journal of Clinical Microbiology, July 2001, p. 2581-2583, Vol. 39, No. 7
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.7.2581-2583.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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