This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Viader-Salvadó, J. M.
Right arrow Articles by Guerrero-Olazarán, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Viader-Salvadó, J. M.
Right arrow Articles by Guerrero-Olazarán, M.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, July 2001, p. 2642-2645, Vol. 39, No. 7
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.7.2642-2645.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Mycolic Acid Index Susceptibility Method for Mycobacterium tuberculosis

José M. Viader-Salvadó,1,* Elvira Garza-González,1 Ramón Valdez-Leal,2 M. de los Angeles del Bosque-Moncayo,2 Rolando Tijerina-Menchaca,1 and Martha Guerrero-Olazarán1

Departamento de Microbiología, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey,1 and Laboratorio Estatal de Salud Pública de los Servicios de Salud de Nuevo León, Guadalupe,2 Nuevo León, México

Received 21 December 2000/Returned for modification 6 February 2001/Accepted 24 April 2001

A rapid drug susceptibility test to measure the susceptibility of Mycobacterium tuberculosis to isoniazid (INH) and rifampin (RIF) using clinical isolates and a newly defined mycolic acid index (MAI) was evaluated. A total of 200 clinical isolates of M. tuberculosis were tested for susceptibility or resistance to INH and RIF by the MAI susceptibility and indirect-proportion methods. Overall, there was agreement between the two methods for 398 (99.5%) of the 400 total tests. Specifically, the sensitivity of the MAI susceptibility method for INH and RIF was 97.6 and 100%, respectively. The specificity and positive predictive value were 100% for both drugs, and the negative predictive value for INH and RIF was 98.3 and 100%, respectively. In conclusion, the MAI susceptibility method described here can be used for rapid drug susceptibility testing of M. tuberculosis clinical isolates within 5 days after clinical isolates are incubated in the presence or absence of an antituberculosis drug.

* Corresponding author. Mailing address: Departamento de Microbiologia, Facultad de Medicina, U.A.N.L., Av. Madero y Dr. E. Aguirre Pequeno, Col. Mitras Centro, 64460 Monterrey, N.L., Mexico. Phone and fax: (528) 329-4050, ext. 2587. E-mail: jviader{at}ccr.dsi.uanl.mx.

Journal of Clinical Microbiology, July 2001, p. 2642-2645, Vol. 39, No. 7
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.7.2642-2645.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Parrish, N., Osterhout, G., Dionne, K., Sweeney, A., Kwiatkowski, N., Carroll, K., Jost, K. C. Jr., Dick, J. (2007). Rapid, Standardized Method for Determination of Mycobacterium tuberculosis Drug Susceptibility by Use of Mycolic Acid Analysis. J. Clin. Microbiol. 45: 3915-3920 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»