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Journal of Clinical Microbiology, July 2001, p. 2648-2651, Vol. 39, No. 7
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.7.2648-2651.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Clarithromycin-Susceptible and -Resistant Helicobacter pylori Isolates with Identical Randomly Amplified Polymorphic DNA-PCR Genotypes Cultured from Single Gastric Biopsy Specimens Prior to Antibiotic Therapy

A. van der Ende,1,* L.-J. van Doorn,2 S. Rooijakkers,1 M. Feller,1 G. N. J. Tytgat,3 and J. Dankert1

Departments of Medical Microbiology1 and Gastroenterology,3 Academic Medical Center, University of Amsterdam, Amsterdam, and Delft Diagnostic Laboratory, Delft,2 The Netherlands

Received 3 January 2001/Returned for modification 28 March 2001/Accepted 20 April 2001

Of the Helicobacter pylori populations from 976 patients, six contained clarithromycin-resistant as well as -susceptible colonies. In each heterogeneous H. pylori population, resistant H. pylori colonies harbored identical 23S ribosomal DNA (rDNA) mutations associated with clarithromycin resistance, while the susceptible H. pylori colonies all had wild-type 23S rDNA. The resistant and susceptible colonies of each of the heterogeneous H. pylori populations had identical randomly amplified polymorphic DNA-PCR genotypes. In conclusion, evaluation of antimicrobial susceptibility can be misinterpreted if only a single colony from the primary H. pylori population is used to test for clarithromycin susceptibility.

* Corresponding author. Mailing address: Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, P.O. Box 22700, 1100 DE Amsterdam, The Netherlands. Phone: 31-20-5664862. Fax: 31-20-6979271. E-mail: a.vanderende{at}amc.uva.nl.

Journal of Clinical Microbiology, July 2001, p. 2648-2651, Vol. 39, No. 7
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.7.2648-2651.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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