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Journal of Clinical Microbiology, July 2001, p. 2660-2662, Vol. 39, No. 7
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.7.2660-2662.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Improved Detection of Methicillin-Resistant Staphylococcus aureus Using Phenyl Mannitol Broth Containing Aztreonam and Ceftizoxime

Heiman Wertheim,1,* Henri A. Verbrugh,1 Cindy van Pelt,1 Peter de Man,2 Alex van Belkum,1 and Margreet C. Vos1

Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center Rotterdam,1 and Department of Medical Microbiology, St. Franciscus Gasthuis,2 Rotterdam, The Netherlands

Received 4 January 2001/Returned for modification 20 March 2001/Accepted 18 April 2001

We tested a phenyl mannitol broth containing ceftizoxime and aztreonam (PHMB+) for detection of methicillin-resistant Staphylococcus aureus (MRSA) with reference MRSA strains and, subsequently, with clinical samples (n = 1,098). All reference MRSA strains induced color change in PHMB+ after 24 to 72 h of incubation. In a clinical setting, 40 MRSA strains were detected with PHMB+, compared with only 23 detected with a routine method. Thus, this selective broth significantly (P < 0.001) improved the rate of MRSA detection.

* Corresponding author. Mailing address: Department of Medical Microbiology and Infectious Diseases, Academic Hospital Rotterdam, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands. Phone: 31.10.4633510. Fax: 31.10.4633875. E-mail: wertheim{at}bacl.azr.nl.

Journal of Clinical Microbiology, July 2001, p. 2660-2662, Vol. 39, No. 7
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.7.2660-2662.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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