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Journal of Clinical Microbiology, July 2001, p. 2663-2667, Vol. 39, No. 7
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.7.2663-2667.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Sensitive Detection of Borrelia burgdorferi Sensu Lato DNA and Differentiation of Borrelia Species by LightCycler PCR

Susanne Mommert, Ralf Gutzmer,* Alexander Kapp, and Thomas Werfel

Department of Dermatology and Allergology, Hannover Medical University, Hannover, Germany

Received 27 November 2000/Returned for modification 4 March 2001/Accepted 8 April 2001

In order to differentiate species within the Borrelia burgdorferi sensu lato complex, LightCyler PCR and melting-curve analysis of the amplicons of two genes with intraspecies variability, the p66 gene and the recA gene, were performed. It was demonstrated that nested LightCycler PCR amplification of p66 is more sensitive in the detection of borrelia DNA than amplification of the recA gene. B. burgdorferi sensu stricto could be differentiated from Borrelia garinii and Borrelia afzelii by melting-curve analysis of the p66 gene amplicon. B. garinii could be differentiated from B. afzelii and B. burgdorferi sensu stricto by melting-curve analysis of the recA gene amplicon. Therefore, the PCRs complement each other in subtyping different Borrelia species, and combined LightCycler PCR and melting-curve analysis of both target genes is a rapid method to distinguish the three species of B. burgdorferi sensu lato.

* Corresponding author. Mailing address: Department of Dermatology and Allergology, Hannover Medical University, Ricklinger Str. 5, D-30449 Hannover, Germany. Phone: 49 511 9246 0. Fax: 49 511 9246 234. E-mail: rgutzmer{at}gmx.de.

Journal of Clinical Microbiology, July 2001, p. 2663-2667, Vol. 39, No. 7
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.7.2663-2667.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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