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Journal of Clinical Microbiology, July 2001, p. 2704-2707, Vol. 39, No. 7
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.7.2704-2707.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Correlations between Molecular Subtyping and Serotyping of Listeria monocytogenes

C. A. Nadon,1 D. L. Woodward,2 C. Young,2 F. G. Rodgers,2 and M. Wiedmann1,*

Cornell University, Ithaca, New York,1 and National Laboratory for Enteric Pathogens, National Microbiology Laboratory, Winnipeg, Manitoba, Canada R3E 3R22

Received 13 December 2000/Returned for modification 22 February 2001/Accepted 24 April 2001

To define relationships between Listeria monocytogenes genetic lineages, ribotypes, and serotypes, 235 L. monocytogenes isolates were characterized by serotyping and automated EcoRI ribotyping. Genetic lineage predicted the following serovar clusters: lineage I, comprising serotypes 1/2b, 3b, 3c, and 4b; lineage II, comprising serotypes 1/2a, 1/2c, and 3a; and lineage III, comprising serotypes 4a and 4c. Some EcoRI ribotypes contained multiple serotypes; a subset of these isolates was further differentiated with PvuII ribotyping. Of the 12 resultant EcoRI-PvuII combination types, only 4 contained multiple serotypes, demonstrating the potential of ribotyping for serotype prediction.

* Corresponding author. Mailing address: Department of Food Science, 412 Stocking Hall, Cornell University, Ithaca, NY 14853. Phone: (607) 254-2838. Fax: (607) 254-4868. E-mail: mw16{at}cornell.edu.

Journal of Clinical Microbiology, July 2001, p. 2704-2707, Vol. 39, No. 7
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.7.2704-2707.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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