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Journal of Clinical Microbiology, July 2001, p. 2713-2716, Vol. 39, No. 7
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.7.2713-2716.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Basis of the Superiority of Cefoperazone Amphotericin Teicoplanin for Isolating Campylobacter upsaliensis from Stools

Catherine Byrne,¹ Deirdre Doherty,¹ Adele Mooney,¹ Margaret Byrne,¹ David Woodward,² Wendy Johnson,² Frank Rodgers,² and Billy Bourke^1,3,*

Children's Research Centre, Our Lady's Hospital for Sick Children,¹ and Department of Paediatrics, The Conway Institute, University College Dublin,³ Dublin 12, Ireland, and National Laboratory for Enteric Pathogens, Canadian Science Centre for Human and Animal Health, Laboratory Centre for Disease Control, Winnipeg, Manitoba, Canada²

Received 27 December 2000/Returned for modification 10 January 2001/Accepted 12 April 2001

The optimum method for isolating Campylobacter upsaliensis from stools has not been clearly defined. In a preliminary study, cefoperazone amphotericin teicoplanin (CAT) selective medium isolated six C. upsaliensis strains which were not detected using modified cefoperazone charcoal deoxycholate (mCCDA). In order to identify the factors that underlie the superiority of CAT over mCCDA for isolating C. upsaliensis, we examined the effect of incubation time and antibiotic content of culture media on the growth of C. upsaliensis isolates using semiquantitative methods. The recovery of a subgroup of C. upsaliensis isolates from seeded stool specimens was also evaluated. Differences in growth of C. upsaliensis on CAT and mCCDA were modest and were not explained by the antibiotic profiles of the two media. Recovery of C. upsaliensis from spiked human feces on CAT was superior to that on mCCDA at lower concentrations of organisms (10³ CFU/ml). We conclude that although CAT is more suitable than mCCDA for the isolation of C. upsaliensis from stools, the superiority of CAT for detecting this organism is not accounted for by the antibiotic composition of the medium.

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^* Corresponding author. Mailing address: The Children's Research Centre, Our Lady's Hospital for Sick Children, Crumlin, Dublin 12, Ireland. Phone: 353-1-4556901. Fax: 353-1-4555307. E-mail: BILLY.BOURKE{at}ucd.ie.

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Journal of Clinical Microbiology, July 2001, p. 2713-2716, Vol. 39, No. 7
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.7.2713-2716.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

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