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Journal of Clinical Microbiology, July 2001, p. 2722-2724, Vol. 39, No. 7
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.7.2722-2724.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Molecular Detection of Human Calicivirus in Young Children Hospitalized with Acute Gastroenteritis in Melbourne, Australia, during 1999

Carl D. Kirkwood* and Ruth F. Bishop

Department of Gastroenterology and Clinical Nutrition, Murdoch Childrens Research Institute, Royal Children's Hospital, Victoria, Australia

Received 21 December 2000/Returned for modification 30 March 2001/Accepted 7 May 2001

Reverse transcription-PCR and sequence analysis identified calciviruses in 32 of 60 stool specimens (negative for other enteric pathogens) obtained from children admitted to our hospital with acute gastroenteritis. The overall annual incidence rate for calcivirus was 9% (32 of 354 children). Molecular analysis identified 30 "Norwalk-like virus" genogroup II (predominantly Lordsdale cluster) and 2 "Sapporo-like virus" strains.

* Corresponding author. Mailing address: Department of Gastroenterology and Clinical Nutrition, Murdoch Childrens Research Institute, Royal Children's Hospital, Flemington Rd., Parkville, Victoria 3052, Australia. Phone: (613) 9345-5060. Fax: (613) 9345-6240. E-mail: Kirkwooc{at}cryptic.rch.unimelb.edu.au.

Journal of Clinical Microbiology, July 2001, p. 2722-2724, Vol. 39, No. 7
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.7.2722-2724.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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