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Journal of Clinical Microbiology, August 2001, p. 2967-2970, Vol. 39, No. 8
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.8.2967-2970.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Detection of Antibodies to U.S. Isolates of Avian Pneumovirus by a Recombinant Nucleocapsid Protein-Based Sandwich Enzyme-Linked Immunosorbent Assay

Baldev R. Gulati,1 Shirin Munir,2 Devi P. Patnayak,1 Sagar M. Goyal,1 and Vivek Kapur2,*

Departments of Veterinary Diagnostic Medicine1 and Veterinary PathoBiology,2 College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota 55108

Received 28 December 2000/Returned for modification 8 April 2001/Accepted 13 May 2001

The nucleocapsid (N) protein of subgroup C (United States-specific) avian pneumovirus (APV/US) was expressed in Escherichia coli, and antibodies to the recombinant N protein were shown to specifically recognize the approx 47-kDa N protein of APV/US by Western immunoblot analysis. The recombinant APV/US N protein was used in a sandwich-capture enzyme-linked immunosorbent assay (ELISA), and the resulting assay was found to be more sensitive and specific than the routine indirect ELISA for the detection of APV/US antibodies in turkey sera.

* Corresponding author. Mailing address: Department of Veterinary PathoBiology, University of Minnesota, 1971 Commonwealth Ave., St. Paul, MN 55108. Phone: (612) 625-7712. Fax: (612) 625-5203. E-mail: vkapur{at}umn.edu.

Journal of Clinical Microbiology, August 2001, p. 2967-2970, Vol. 39, No. 8
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.8.2967-2970.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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  • Alvarez, R., Njenga, M. K., Scott, M., Seal, B. S. (2004). Development of a Nucleoprotein-Based Enzyme-Linked Immunosorbent Assay Using a Synthetic Peptide Antigen for Detection of Avian Metapneumovirus Antibodies in Turkey Sera. CVI 11: 245-249 [Abstract] [Full Text]  
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