This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Luo, G.
Right arrow Articles by Yau, J. Y. Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Luo, G.
Right arrow Articles by Yau, J. Y. Y.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, August 2001, p. 2971-2974, Vol. 39, No. 8
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.8.2971-2974.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Candida Species Exhibit Differential In Vitro Hemolytic Activities

Gang Luo,1,2 Lakshman P. Samaranayake,1,* and Joyce Y. Y. Yau1

Oral Bio-Sciences, Faculty of Dentistry, The University of Hong Kong, Hong Kong, Special Administrative Region,1 and College of Stomatology, West China University of Medical Sciences, Chengdu,2 People's Republic of China

Received 21 March 2001/Returned for modification 2 May 2001/Accepted 3 June 2001

A total of 80 Candida isolates representing 14 species were examined for their respective responses to an in vitro hemolytic test. A modification of a previously described plate assay system where the yeasts are incubated on glucose (3%)-enriched sheep blood agar in a carbon dioxide (5%)-rich environment for 48 h was used to evaluate the hemolytic activity. A group of eight Candida species which included Candida albicans (15 isolates), C. dubliniensis (2), C. kefyr (2), C. krusei (4), C. zeylanoides (1), C. glabrata (34), C. tropicalis (5), and C. lusitaniae (2) demonstrated both alpha and beta hemolysis at 48 h postinoculation. Only alpha hemolysis was detectable in four Candida species, viz., C. famata (3), C. guilliermondii (4), C. rugosa (1), and C. utilis (1), while C. parapsilosis (5) and C. pelliculosa (1) failed to demonstrate any hemolytic activity after incubation for 48 h or longer. This is the first study to demonstrate the variable expression profiles of hemolysins by different Candida species.

* Corresponding author. Mailing address: Oral Bio-Sciences, Faculty of Dentistry, The University of Hong Kong, 34 Hospital Rd., Hong Kong. Phone: 852-2859 0480. Fax: 852-2547 6133. E-mail: lakshman{at}hkucc.hku.hk.

Journal of Clinical Microbiology, August 2001, p. 2971-2974, Vol. 39, No. 8
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.8.2971-2974.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Rosa, E. A. R., Rached, R. N., Ignacio, S. A., Rosa, R. T., Jose da Silva, W., Yau, J. Y. Y., Samaranayake, L. P. (2008). Phenotypic evaluation of the effect of anaerobiosis on some virulence attributes of Candida albicans. J Med Microbiol 57: 1277-1281 [Abstract] [Full Text]  
  • Chaffin, W. L. (2008). Candida albicans Cell Wall Proteins. Microbiol. Mol. Biol. Rev. 72: 495-544 [Abstract] [Full Text]  
  • Tsang, C. S. P., Chu, F. C. S., Leung, W. K., Jin, L. J., Samaranayake, L. P., Siu, S. C. (2007). Phospholipase, proteinase and haemolytic activities of Candida albicans isolated from oral cavities of patients with type 2 diabetes mellitus. J Med Microbiol 56: 1393-1398 [Abstract] [Full Text]  
  • Weaver, L. K., Hintz-Goldstein, K. A., Pioli, P. A., Wardwell, K., Qureshi, N., Vogel, S. N., Guyre, P. M. (2006). Pivotal Advance: Activation of cell surface Toll-like receptors causes shedding of the hemoglobin scavenger receptor CD163. J. Leukoc. Biol. 80: 26-35 [Abstract] [Full Text]  
  • Knight, S. A. B., Vilaire, G., Lesuisse, E., Dancis, A. (2005). Iron Acquisition from Transferrin by Candida albicans Depends on the Reductive Pathway. Infect. Immun. 73: 5482-5492 [Abstract] [Full Text]  
  • Samaranayake, Y H, Dassanayake, R S, Jayatilake, J A., Cheung, B P., Yau, J Y., Yeung, K W., Samaranayake, L P (2005). Phospholipase B enzyme expression is not associated with other virulence attributes in Candida albicans isolates from patients with human immunodeficiency virus infection. J Med Microbiol 54: 583-593 [Abstract] [Full Text]  
  • Pendrak, M. L., Yan, S. S., Roberts, D. D. (2004). Hemoglobin Regulates Expression of an Activator of Mating-Type Locus {alpha} Genes in Candida albicans. Eukaryot Cell 3: 764-775 [Abstract] [Full Text]  
  • Pendrak, M. L., Chao, M. P., Yan, S. S., Roberts, D. D. (2004). Heme Oxygenase in Candida albicans Is Regulated by Hemoglobin and Is Necessary for Metabolism of Exogenous Heme and Hemoglobin to {alpha}-Biliverdin. J. Biol. Chem. 279: 3426-3433 [Abstract] [Full Text]  
  • Rodrigues, A. G., Pina-Vaz, C., Costa-de-Oliveira, S., Tavares, C. (2003). Expression of Plasma Coagulase among Pathogenic Candida Species. J. Clin. Microbiol. 41: 5792-5793 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»