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Journal of Clinical Microbiology, August 2001, p. 2987-2990, Vol. 39, No. 8
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.8.2987-2990.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Mutations in the rpoB Gene of Multidrug-Resistant Mycobacterium tuberculosis Clinical Isolates from India

Cheruvu Mani, N. Selvakumar,* Sujatha Narayanan, and P. R. Narayanan

Tuberculosis Research Centre, Chetput, Chennai 600 031, Tamil Nadu, India

Received 27 November 2000/Returned for modification 28 March 2001/Accepted 23 April 2001

Mutations in the 81-bp rifampin resistance-determining region (RRDR) of the rpoB gene were analyzed by DNA sequencing of 50 Mycobacterium tuberculosis clinical isolates (44 resistant and 6 sensitive) from various parts of India. Fifty-three mutations of 18 different kinds, 17 point mutations and one deletion, were observed in 43 of 44 resistant isolates. Three novel mutations and three new alleles within the RRDR, along with two novel mutations outside the RRDR, are reported in this study.

* Corresponding author. Mailing address: Tuberculosis Research Centre (ICMR), Mayor V. R. Ramanathan Rd., Chetput, Chennai 600 031, Tamil Nadu, India. Phone: 91-(44)-8265425. Fax: 91-(44)-8262137. E-mail: icmrtrc{at}vsnl.com.

Journal of Clinical Microbiology, August 2001, p. 2987-2990, Vol. 39, No. 8
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.8.2987-2990.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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