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Journal of Clinical Microbiology, August 2001, p. 2991-2994, Vol. 39, No. 8
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.8.2991-2994.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Possible Approach for Serodiagnosis of Ascariasis by Evaluation of Immunoglobulin G4 Response Using Ascaris lumbricoides Somatic Antigen

Tanusree Bhattacharyya,1 Amal Santra,2 Debendra N. Guha Majumder,2 and Bishnu P. Chatterjee1,*

Department of Biological Chemistry, Indian Association for the Cultivation of Science, Jadavpur, Calcutta 700 032,1 and Department of Gastroenterology, Institute of Post Graduate Medical Education and Research, Calcutta 700 020,2 India

Received 1 March 2001/Returned for modification 3 April 2001/Accepted 30 May 2001

Somatic antigen of Ascaris lumbricoides was purified to homogeneity (molecular mass, 34 kDa) by ammonium sulfate fractionation and successive chromatographic procedures, namely, gel permeation, ion exchange, and high-performance gel permeation liquid chromatographies. The antigen showed strong binding with immunoglobulin G (IgG) in Ascaris-infested patients and was cross-reactive with IgE and IgG in patients infested with other nematodes. It reacted specifically with IgG4 (P < 0.001) in 63 Ascaris-infested patients, which represented 65% of the total IgG response, though cross-reactivity with IgG1, IgG2, and IgG3 subclasses was observed, indicating the unique specificity of this test system and its potential utility in the serodiagnosis of ascariasis.

* Corresponding author. Mailing address: Department of Biological Chemistry, Indian Association for the Cultivation of Science, 2A&B, Raja S. C. Mallik Rd., Jadavpur, Calcutta 700 032, India. Phone: 91-33-473-4971. Fax: 91-33-473-2805. E-mail: bcbpc{at}mahendra.iacs.res.in.

Journal of Clinical Microbiology, August 2001, p. 2991-2994, Vol. 39, No. 8
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.8.2991-2994.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.




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