This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow An erratum has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bertin, Y.
Right arrow Articles by Martin, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bertin, Y.
Right arrow Articles by Martin, C.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, September 2001, p. 3060-3065, Vol. 39, No. 9
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.9.3060-3065.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Stx2 Subtyping of Shiga Toxin-Producing Escherichia coli Isolated from Cattle in France: Detection of a New Stx2 Subtype and Correlation with Additional Virulence Factors

Yolande Bertin,1,* Karima Boukhors,1 Nathalie Pradel,2 Valerie Livrelli,2 and Christine Martin1

Laboratoire de Microbiologie, Centre de Recherche INRA de Clermont-Ferrand-Theix, 63122 St-Genès Champanelle,1 and Groupe de Recherche Pathogénie Bactérienne Intestinale, Faculté de Pharmacie, Universite d'Auvergne, Clermont-Ferrand,2 France

Received 27 December 2000/Returned for modification 8 April 2001/Accepted 17 June 2001

At least 11 Stx2 variants produced by Shiga toxin-producing Escherichia coli (STEC) isolated from patients and animals have been described. The Stx2 subtyping of STEC isolated from healthy cows positive for stx2 (n = 104) or stx2 and stx1 (n = 63) was investigated. Stx2vh-b, Stx2 (renamed Stx2-EDL933), and Stx2vh-a were the subtypes mostly detected among the bovine isolates (39.5, 39, and 25.5%, respectively). Stx2e was not present, and subtypes included in the Stx2d group (Stx2d-OX3a, Stx2d-O111, and Stx2d-Ount) were found infrequently among the isolates examined (8.5%). A combination of two distinct Stx2 subtypes was observed among 23.5% of the strains. For the first time, a combination of three subtypes (Stx2-EDL933/Stx2vh-b/Stx2d and Stx2vh-a/Stx2vh-b/Stx2d) was detected (3.5% of the isolates). In addition, bovine STEC harboring stx1 and one or two stx2 genes appeared highly cytotoxic toward Vero cells. A new Stx2 subtype (Stx2-NV206), present among 14.5% of the isolates, showed high cytotoxicity for Vero cells. Two amino acid residues (Ser-291 and Glu-297) important for the activation of Stx2 by human intestinal mucus were conserved on the Stx2-NV206 A subunit. The gene encoding Ehx enterohemolysin was prominent among STEC harboring stx2-EDL933 alone (78%) or a combination of stx2-EDL933 and stx2vh-b (85%). In addition, Stx2-EDL933 and/or Stx2vh-b subtypes were highly associated with other putative virulence factors such as Stx1 and EspP extracellular serine protease, but not with EAST1 enterotoxin.

* Corresponding author. Mailing address: Laboratoire de Microbiologie, Centre de Recherche INRA de Clermont-Ferrand-Theix, 63122 St-Genes Champanelle, France. Phone: (33) 4 73 62 42 42. Fax: (33) 4 73 62 45 81. E-mail: bertin{at}clermont.inra.fr.

Journal of Clinical Microbiology, September 2001, p. 3060-3065, Vol. 39, No. 9
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.9.3060-3065.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Frohlich, J., Baljer, G., Menge, C. (2009). Maternally and Naturally Acquired Antibodies to Shiga Toxins in a Cohort of Calves Shedding Shiga-Toxigenic Escherichia coli. Appl. Environ. Microbiol. 75: 3695-3704 [Abstract] [Full Text]  
  • Zheng, J., Cui, S., Teel, L. D., Zhao, S., Singh, R., O'Brien, A. D., Meng, J. (2008). Identification and Characterization of Shiga Toxin Type 2 Variants in Escherichia coli Isolates from Animals, Food, and Humans. Appl. Environ. Microbiol. 74: 5645-5652 [Abstract] [Full Text]  
  • Pradel, N., Bertin, Y., Martin, C., Livrelli, V. (2008). Molecular Analysis of Shiga Toxin-Producing Escherichia coli Strains Isolated from Hemolytic-Uremic Syndrome Patients and Dairy Samples in France. Appl. Environ. Microbiol. 74: 2118-2128 [Abstract] [Full Text]  
  • de Sablet, T., Bertin, Y., Vareille, M., Girardeau, J.-P., Garrivier, A., Gobert, A. P., Martin, C. (2008). Differential expression of stx2 variants in Shiga toxin-producing Escherichia coli belonging to seropathotypes A and C. Microbiology 154: 176-186 [Abstract] [Full Text]  
  • Beutin, L., Miko, A., Krause, G., Pries, K., Haby, S., Steege, K., Albrecht, N. (2007). Identification of Human-Pathogenic Strains of Shiga Toxin-Producing Escherichia coli from Food by a Combination of Serotyping and Molecular Typing of Shiga Toxin Genes. Appl. Environ. Microbiol. 73: 4769-4775 [Abstract] [Full Text]  
  • Persson, S., Olsen, K. E. P., Ethelberg, S., Scheutz, F. (2007). Subtyping Method for Escherichia coli Shiga Toxin (Verocytotoxin) 2 Variants and Correlations to Clinical Manifestations. J. Clin. Microbiol. 45: 2020-2024 [Abstract] [Full Text]  
  • Chapman, T. A., Wu, X.-Y., Barchia, I., Bettelheim, K. A., Driesen, S., Trott, D., Wilson, M., Chin, J. J.-C. (2006). Comparison of Virulence Gene Profiles of Escherichia coli Strains Isolated from Healthy and Diarrheic Swine.. Appl. Environ. Microbiol. 72: 4782-4795 [Abstract] [Full Text]  
  • Loukiadis, E., Kerouredan, M., Beutin, L., Oswald, E., Brugere, H. (2006). Characterization of Shiga Toxin Gene (stx)-Positive and Intimin Gene (eae)-Positive Escherichia coli Isolates from Wastewater of Slaughterhouses in France.. Appl. Environ. Microbiol. 72: 3245-3251 [Abstract] [Full Text]  
  • Geue, L., Selhorst, T., Schnick, C., Mintel, B., Conraths, F. J. (2006). Analysis of the Clonal Relationship of Shiga Toxin-Producing Escherichia coli Serogroup O165:H25 Isolated from Cattle.. Appl. Environ. Microbiol. 72: 2254-2259 [Abstract] [Full Text]  
  • Girardeau, J. P., Dalmasso, A., Bertin, Y., Ducrot, C., Bord, S., Livrelli, V., Vernozy-Rozand, C., Martin, C. (2005). Association of Virulence Genotype with Phylogenetic Background in Comparison to Different Seropathotypes of Shiga Toxin-Producing Escherichia coli Isolates. J. Clin. Microbiol. 43: 6098-6107 [Abstract] [Full Text]  
  • Sheng, H., Davis, M. A., Knecht, H. J., Hancock, D. D., Van Donkersgoed, J., Hovde, C. J. (2005). Characterization of a Shiga Toxin-, Intimin-, and Enterotoxin Hemolysin-Producing Escherichia coli ONT:H25 Strain Commonly Isolated from Healthy Cattle. J. Clin. Microbiol. 43: 3213-3220 [Abstract] [Full Text]  
  • De Baets, L., Van der Taelen, I., De Filette, M., Pierard, D., Allison, L., De Greve, H., Hernalsteens, J.-P., Imberechts, H. (2004). Genetic Typing of Shiga Toxin 2 Variants of Escherichia coli by PCR-Restriction Fragment Length Polymorphism Analysis. Appl. Environ. Microbiol. 70: 6309-6314 [Abstract] [Full Text]  
  • Garcia-Aljaro, C., Muniesa, M., Jofre, J., Blanch, A. R. (2004). Prevalence of the stx2 Gene in Coliform Populations from Aquatic Environments. Appl. Environ. Microbiol. 70: 3535-3540 [Abstract] [Full Text]  
  • Vernozy-Rozand, C., Montet, M. P., Bertin, Y., Trably, F., Girardeau, J. P., Martin, C., Livrelli, V., Beutin, L. (2004). Serotyping, stx2 Subtyping, and Characterization of the Locus of Enterocyte Effacement Island of Shiga Toxin-Producing Escherichia coli and E. coli O157:H7 Strains Isolated from the Environment in France. Appl. Environ. Microbiol. 70: 2556-2559 [Abstract] [Full Text]  
  • Ferens, W. A., Grauke, L. J., Hovde, C. J. (2004). Shiga Toxin 1 Targets Bovine Leukemia Virus-Expressing Cells. Infect. Immun. 72: 1837-1840 [Abstract] [Full Text]  
  • Gobius, K. S., Higgs, G. M., Desmarchelier, P. M. (2003). Presence of Activatable Shiga Toxin Genotype (stx2d) in Shiga Toxigenic Escherichia coli from Livestock Sources. J. Clin. Microbiol. 41: 3777-3783 [Abstract] [Full Text]  
  • Brett, K. N., Hornitzky, M. A., Bettelheim, K. A., Walker, M. J., Djordjevic, S. P. (2003). Bovine Non-O157 Shiga Toxin 2-Containing Escherichia coli Isolates Commonly Possess stx2-EDL933 and/or stx2vhb Subtypes. J. Clin. Microbiol. 41: 2716-2722 [Abstract] [Full Text]  
  • Brett, K. N., Ramachandran, V., Hornitzky, M. A., Bettelheim, K. A., Walker, M. J., Djordjevic, S. P. (2003). stx1c Is the Most Common Shiga Toxin 1 Subtype among Shiga Toxin-Producing Escherichia coli Isolates from Sheep but Not among Isolates from Cattle. J. Clin. Microbiol. 41: 926-936 [Abstract] [Full Text]  
  • Basu, I., Ferens, W. A., Stone, D. M., Hovde, C. J. (2003). Antiviral Activity of Shiga Toxin Requires Enzymatic Activity and Is Associated with Increased Permeability of the Target Cells. Infect. Immun. 71: 327-334 [Abstract] [Full Text]  
  • Reischl, U., Youssef, M. T., Kilwinski, J., Lehn, N., Zhang, W. L., Karch, H., Strockbine, N. A. (2002). Real-Time Fluorescence PCR Assays for Detection and Characterization of Shiga Toxin, Intimin, and Enterohemolysin Genes from Shiga Toxin-Producing Escherichia coli. J. Clin. Microbiol. 40: 2555-2565 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»