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Journal of Clinical Microbiology, September 2001, p. 3356-3359, Vol. 39, No. 9
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.9.3356-3359.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Isolation and Characterization of a Species-Specific DNA Fragment for Identification of Candida (Torulopsis) glabrata by PCR

Karsten Becker,1,* Dörte Badehorn,1 Birgit Keller,2 Martina Schulte,1 Karl Heinz Böhm,2 Georg Peters,1 and Wolfgang Fegeler1

Institute of Medical Microbiology, University of Münster, D-48149 Münster,1 and Department of Microbiology and Infectious Diseases, School of Veterinary Medicine, D-30173 Hannover,2 Germany

Received 9 February 2001/Returned for modification 9 May 2001/Accepted 14 June 2001

A PCR specific for Candida glabrata that amplifies a mitochondrial rRNA gene fragment was developed by analysis of C. glabrata-specific agarose gel bands, which were generated by arbitrarily primed PCR. The expected PCR product was successfully amplified with genomic DNA from 95 C. glabrata isolates but not from a number of other fungal isolates.

* Corresponding author. Mailing address: Hospital and Clinics, Institute of Medical Microbiology, University of Münster, D-48149 Münster, Germany. Phone: (49) 251 83-55360. Fax: (49) 251 83-55350. E-mail: kbecker{at}uni-muenster.de.

Journal of Clinical Microbiology, September 2001, p. 3356-3359, Vol. 39, No. 9
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.9.3356-3359.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Becker, K., Almasri, A. S., von Eiff, C., Peters, G., Heilmann, C., Fegeler, W. (2007). Systematic Survey of Nonspecific Agglutination by Candida spp. in Latex Assays. J. Clin. Microbiol. 45: 1315-1318 [Abstract] [Full Text]  


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