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Journal of Clinical Microbiology, September 2001, p. 3409-3413, Vol. 39, No. 9
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.9.3409-3413.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Murine Monoclonal Antibodies against Escherichia coli O4 Lipopolysaccharide and H5 Flagellin

Mildred Rivera-Betancourt and James E. Keen*

United States Department of Agriculture, Agricultural Research Service, Roman L. Hruska U.S. Meat Animal Research Center (MARC), Clay Center, Nebraska 68933

Received 22 December 2000/Returned for modification 22 March 2001/Accepted 17 June 2001

Two murine monoclonal antibodies (MAb), 2C5-F10 and 8D1-H10, reactive with Escherichia coli O4 and H5 antigens, respectively, were generated and characterized. Enzyme immunoassays and immunoblots demonstrated that MAb 2C5-F10 reacted specifically with lipopolysaccharide O antigen of E. coli O4 isolates, while MAb 8D1-H10 reacted with E. coli strains expressing H5 flagella.

* Corresponding author. Mailing address: USDA, ARS, U.S. Meat Animal Research Center, P.O. Box 166, State Spur 18D, Clay Center, NE 68933. Phone: (402) 762-4343. Fax: (402) 762-4375. E-mail: keen{at}emailmarc.usda.gov.

Journal of Clinical Microbiology, September 2001, p. 3409-3413, Vol. 39, No. 9
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.9.3409-3413.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.




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