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Journal of Clinical Microbiology, January 2002, p. 111-116, Vol. 40, No. 1
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.1.111-116.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Simultaneous Detection of Measles Virus, Rubella Virus, and Parvovirus B19 by Using Multiplex PCR

María del Mar Mosquera,1* Fernando de Ory,1 Mónica Moreno,2 and Juan E. Echevarría1

Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda,1 Centro de Attencion Primaria de Galapagar, Madrid, Spain2

Received 26 July 2001/ Returned for modification 19 September 2001/ Accepted 15 October 2001

We describe here a multiplex reverse transcription-PCR (RTMNPCR) assay designed to detect and differentiate measles virus, rubella virus, and parvovirus B19. Serial dilution experiments with vaccine strains that compared cell culture isolation of measles in B95 cells and rubella in RK13 cells showed sensitivity rates of 0.004 50% tissue culture infective dose (TCID50) for measles virus and 0.04 TCID50 for rubella virus. This RTMNPCR can detect as few as 10 molecules for measles virus and rubella virus and one molecule for parvovirus B19 in dilution experiments with plasmids containing inserts of the primary reaction amplification products. Five pharyngeal exudates from measles patients and 2 of 15 cerebrospinal fluid samples from measles-related encephalitis were found to be positive for measles virus by this RTMNPCR. A total of 3 of 27 pharyngeal exudates from vaccinated children and 2 pharyngeal exudates, plus one urine sample from a case of congenital rubella syndrome, were found to be positive for rubella virus by RTMNPCR, whereas 16 of 19 sera from patients with erythema infectiosum were determined to be positive for parvovirus B19 by RTMNPCR. In view of these results, we can assess that this method is a useful tool in the diagnosis of these three viruses and could be used as an effective surveillance tool in measles eradication programs.

* Corresponding author. Mailing address: Centro Nacional de Microbiología, Instituto de Salud Carlos III, Carretera Majadahonda-Pozuelo s/n, 28220 Majadahonda, Spain. Phone: 34-1-5097901. Fax: 34-1-5097966. E-mail: mmosquera{at}isciii.es.

Journal of Clinical Microbiology, January 2002, p. 111-116, Vol. 40, No. 1
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.1.111-116.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Martinez-Torres, A. O., Mosquera, M. M., Sanz, J. C., Ramos, B., Echevarria, J. E. (2009). Phylogenetic Analysis of Rubella Virus Strains from an Outbreak in Madrid, Spain, from 2004 to 2005. J. Clin. Microbiol. 47: 158-163 [Abstract] [Full Text]  
  • Lopez-Alvarez, M. R., Martinez-Sanchez, M. V., Salgado-Cecilia, M. G., Campillo, J. A., Heine-Suner, D., Villar-Permuy, F., Fuster, J. L., Bas, A., Gil-Herrera, J., Muro, M., Garcia-Alonso, A. M., Alvarez-Lopez, M. R., Minguela, A. (2009). Association of Monoclonal Expansion of Epstein-Barr Virus-Negative CD158a+ NK Cells Secreting Large Amounts of Gamma Interferon with Hemophagocytic Lymphohistiocytosis. CVI 16: 142-145 [Abstract] [Full Text]  
  • Mosquera, M. M., de Ory, F., Gallardo, V., Cuenca, L., Morales, M., Sanchez-Yedra, W., Cabezas, T., Hernandez, J. M., Echevarria, J. E. (2005). Evaluation of Diagnostic Markers for Measles Virus Infection in the Context of an Outbreak in Spain. J. Clin. Microbiol. 43: 5117-5121 [Abstract] [Full Text]  


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