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Journal of Clinical Microbiology, January 2002, p. 133-139, Vol. 40, No. 1
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.1.133-139.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Molecular Epidemiology of Astrovirus Infection in Barcelona, Spain

Susana Guix,1 Santiago Caballero,1 Cristina Villena,1 Rosa Bartolomé,2 Cristina Latorre,3 Nuria Rabella,4 Maria Simó,5 Albert Bosch,1* and Rosa M. Pintó1

Enteric Virus Laboratory, Department of Microbiology, University of Barcelona, Barcelona,1 Laboratory of Microbiology, Hospital de la Vall d’Hebron,2 Laboratory of Microbiology, Hospital de Sant Joan de Deu,3 Laboratory of Microbiology, Hospital de la Santa Creu i Sant Pau,4 Laboratory of Microbiology, Consorci Sanitari de Terrassa5

Received 26 February 2001/ Returned for modification 18 August 2001/ Accepted 3 November 2001

A 3-year study involving 2,347 gastroenteritis samples was conducted to determine the prevalence, time distribution, and medical significance of human astrovirus infection in Barcelona, Spain. The overall incidence of astrovirus was found to be 4.9%. Mixed infections with other enteric agents were detected in 17.2% of all astrovirus-positive samples. During the 3-year period, the highest astrovirus incidence was reported in the winter months, although infections also occurred in summer. The peak detection rate was observed in children between 2 and 4 years of age. Overall, HAstV-1 was the most prevalent type, followed by HAstV-4, HAstV-3, HAstV-8, and HAstV-2. HAstV-5, HAstV-6, and HAstV-7 were not detected during these 3 years. From our serotype data for each age group, we observed that HAstV-1, HAstV-2, and HAstV-3 affected mostly children younger than 3 years of age, while HAstV-4 and HAstV-8 had a greater impact in older children. Genetic variability was analyzed between astroviruses isolated in Barcelona and strains isolated in other parts of the world. A fourth lineage was described for HAstV-1, most likely due to the large number of assayed samples, which may also explain the high level of genetic variability observed in the astrovirus isolates.

* Corresponding author. Mailing address: Department of Microbiology, University of Barcelona, Avda Diagonal 645, 08028 Barcelona, Spain. Phone: (34) 934034620. Fax: (34) 934034629. E-mail: albert{at}porthos.bio.ub.es.

Journal of Clinical Microbiology, January 2002, p. 133-139, Vol. 40, No. 1
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.1.133-139.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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