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Journal of Clinical Microbiology, January 2002, p. 262-264, Vol. 40, No. 1
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.1.262-264.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Accurate, Noninvasive Detection of Helicobacter pylori DNA from Stool Samples: Potential Usefulness for Monitoring Treatment

Anthony P. Shuber,1 Jennifer J. Ascaño,2 Kevin A. Boynton,1 Anastasia Mitchell,2 Henry F. Frierson Jr.,3 Wa’el El-Rifai,2 and Steven M. Powell2*

Applied Research Group, Exact Sciences Corporation, Maynard, Massachusetts 01754,1 Division of Gastroenterology/Hepatology,2 Department of Pathology, University of Virginia Health System, Charlottesville, Virginia 229083

Received 12 July 2001/ Returned for modification 20 August 2001/ Accepted 5 October 2001

A novel DNA assay demonstrating sensitive and accurate detection of Helicobacter pylori from stool samples is reported. Moreover, in three individuals tested for therapeutic response, the assay showed the disappearance of H. pylori DNA during treatment. Thus, this noninvasive molecular biology-based assay has the potential to be a powerful diagnostic tool given its ability to specifically identify H. pylori DNA.

* Corresponding author. Mailing address: Department of Medicine, Division of Gastroenterology/Hepatology, University of Virginia Health Sciences Center, Box 800708, Charlottesville, VA 22908-0708. Phone: (804) 243-2718. Fax: (804) 243-0491. E-mail: smp8n{at}virginia.edu.

Journal of Clinical Microbiology, January 2002, p. 262-264, Vol. 40, No. 1
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.1.262-264.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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