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Journal of Clinical Microbiology, January 2002, p. 281-283, Vol. 40, No. 1
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.1.281-283.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Evaluation of a Strand Displacement Amplification Assay (BD ProbeTec-SDA) for Detection of Neisseria gonorrhoeae in Urine Specimens

Deniz Akduman,1 Josephine M. Ehret,1,2 Kimberly Messina,1 Sharron Ragsdale,1 and Franklyn N. Judson1,2,3*

Denver Public Health Department,1 Departments of Medicine,2 Preventive Medicine, University of Colorado Health Sciences Center, Denver, Colorado3

Received 13 June 2001/ Returned for modification 25 July 2001/ Accepted 15 October 2001

The performance of a strand displacement amplification assay (the BDProbeTec-SDA assay) in detecting Neisseria gonorrhoeae in urine specimens was evaluated. When performed under stringent quality control conditions, the BDProbeTec-SDA assay is a sensitive, specific, and efficient method for the screening of large numbers of noninvasively obtained specimens. Because the predictive value of an assay is a function of the prevalence of the disease, culture confirmation is needed for samples with positive results from populations in which the prevalence of a disease is low or in situations in which false-positive results may have important medical, psychosocial, or medicolegal consequences.

* Corresponding author. Mailing address: Denver Public Health, 605 Bannock St., Denver, CO 80204-4507. Phone: (303) 436-7200. Fax: (303) 436-7211. E-mail: Frank.Judson{at}dhha.org.

Journal of Clinical Microbiology, January 2002, p. 281-283, Vol. 40, No. 1
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.1.281-283.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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