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Journal of Clinical Microbiology, October 2002, p. 3602-3605, Vol. 40, No. 10
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.10.3602-3605.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Identification of Candida glabrata by a 30-Second Trehalase Test

A.-M. Freydière,1* F. Parant,1 F. Noel-Baron,1 M. Crepy,1 A. Treny,1 H. Raberin,2 A. Davidson,3 and F. C. Odds3

Laboratoire de Microbiologie, Hopital Debrousse, Hospices Civils de Lyon, 69322 Lyon cedex 05,1 Laboratoire de Mycologie, CHU Hôpital Nord, 42055 St. Etienne cedex 02, France,2 Department of Molecular and Cell Biology, Institute of Medical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, United Kingdom3

Received 15 January 2002/ Returned for modification 7 April 2002/ Accepted 12 July 2002

Rapid (30-s) trehalase tests done with material from colonies of 482 yeasts suspended in a drop of trehalose solution on a commercially supplied glucose test strip were positive for 225 (99.1%) of 227 Candida glabrata isolates grown on either of two differential media, Candida ID medium or CandiSelect medium. The test was positive for only 3 (1.2%) and 12 (4.7%) of 255 isolates of other medically important yeast species grown on the same two media, respectively. A rapid maltase test done with a subset of 255 yeast isolates was negative for all but 1 of 64 trehalase-positive C. glabrata isolates, raising the specificity of the rapid testing for C. glabrata to 98.4 to 100%, depending on the isolation medium used. Rapid trehalase and maltase tests done independently in two laboratories with 217 yeast isolates showed sensitivities of 96.0 to 98.0% and specificities of 98.2 to 99.4% for identification of C. glabrata from colonies grown on Candida ID medium. The specificity was much lower because of frequent false-positive trehalose test results when the source of colonies was Sabouraud agar formulated with 4% glucose. We conclude that direct recognition of C. albicans as blue colonies on Candida ID isolation medium coupled with the performance of the 30-s trehalase and maltase tests for C. glabrata among the white colonies on this medium will allow the rapid presumptive identification of the two yeast species most commonly encountered in clinical samples.

* Corresponding author. Mailing address: Laboratoire de Microbiologie, Hôpital Debrousse, Hospices Civils de Lyon, 29 Rue Soeur Bouvier, 69322 Lyon cedex 05, France. Phone: (33) 4 7238 5816. Fax: (33) 4 7238 5535. E-mail: anne-marie.freydiere{at}chu-lyon.fr.

Journal of Clinical Microbiology, October 2002, p. 3602-3605, Vol. 40, No. 10
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.10.3602-3605.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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