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Journal of Clinical Microbiology, October 2002, p. 3660-3665, Vol. 40, No. 10
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.10.3660-3665.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Genotypes of Invasive Pneumococcal Isolates Recently Recovered from Italian Patients

Giordano Dicuonzo,1* Giovanni Gherardi,1 Robert E. Gertz,2 Fabio D'Ambrosio,3 Antonio Goglio,4 Giulia Lorino,5 Simona Recchia,3 Annalisa Pantosti,3 and Bernard Beall2

Dipartimento di Medicina di Laboratorio e Microbiologia, Università Campus Bio-Medico,1 Laboratorio di Batteriologia e Micologia Medica, Istituto Superiore di Sanità,3 Laboratorio di Microbiologia, Università La Sapienza, Rome,5 Laboratorio di Microbiologia, Ospedali Riuniti, Bergamo, Italy,4 National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia2

Received 28 May 2002/ Returned for modification 21 June 2002/ Accepted 12 July 2002

We examined 73 recent invasive pneumococcal isolates within selected areas of Italy for genotypic variability. Thirty-three genomic macrorestriction types were found, three of which represented multiple serotypes. Restriction fragment patterns of pbp2b, pbp2x, and pspA were conserved within the majority of isolates that shared macrorestriction types. Of the nine macrorestriction types found among the 22 penicillin-nonsusceptible Streptococus pneumoniae (PNSP) isolates, seven comprised isolates with allelic profiles showing five to seven allelic matches to profiles in the multilocus sequence typing database (www.mlst.net); however, three of the seven profiles represented serotypes not previously associated with these clonal clusters. Two PNSP macrorestriction types represented new clones with unique allelic profiles. Allelic profiles obtained from isolates of 3 of the 25 macrorestriction types found among the 51 penicillin-susceptible S. pneumoniae (PSSP) isolates were closely related to previously described profiles. One PSSP isolate was a novel type 24F isolate related to the multiresistant clone France9V-3. This work reports new PNSP strains and new serotype-clone associations.

* Corresponding author. Mailing address: Dipartimento di Medicina di Laboratorio e Microbiologia, Università Campus Bio-Medico, Via Emilio Longoni 47, 00155 Rome, Italy. Phone: 390622541790. Fax: 390622541791. E-mail: g.dicuonzo{at}unicampus.it.

Journal of Clinical Microbiology, October 2002, p. 3660-3665, Vol. 40, No. 10
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.10.3660-3665.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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